Fraser Christopher S, Hershey John W B
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 947020, USA.
J Biol. 2005;4(2):8. doi: 10.1186/jbiol27. Epub 2005 Jun 27.
Translocation of peptidyl-tRNA and mRNA within the ribosome during protein synthesis is promoted by the elongation factor EF-G and by the hydrolysis of GTP. A new study reports that EF-G binds to ribosomes as an EF-G.GDP complex and that GTP is exchanged for GDP on the ribosome. Together with cryo-electron microscopy, this unexpected finding helps clarify the role of GTP in translocation.
在蛋白质合成过程中，肽基 - tRNA和mRNA在核糖体中的易位是由延伸因子EF - G以及GTP水解所促进的。一项新的研究报告称，EF - G以EF - G·GDP复合物的形式与核糖体结合，并且在核糖体上GTP会与GDP发生交换。结合冷冻电子显微镜技术，这一意外发现有助于阐明GTP在易位过程中的作用。
