Lau Stanley C K, Tsoi Mandy M Y, Li Xiancui, Dobretsov Sergey, Plakhotnikova Yulia, Wong Po-Keung, Qian Pei-Yuan
Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Department of Biology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1593-1596. doi: 10.1099/ijs.0.63638-0.
A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006(T)) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006(T) did not have flagella and were non-motile. Colonies were pale orange in colour, 2-4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006(T) was heterotrophic, strictly aerobic and required NaCl for growth (2.0-6.0%). It grew at pH 5.0-10.0 and between 12 and 44 degrees C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006(T) within the genus Pseudoalteromonas of the gamma-subclass of the Proteobacteria. The DNA G+C content is 40.6 mol% and the dominant fatty acids were 12:0 3-OH, 14:0, 15:0 iso 2-OH, 16:0, 16:1omega7, 17:1omega8 and 18:1omega7 (altogether representing 75.9% of the total). These data supported the affiliation of UST010723-006(T) to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95.4 to 96.8%. UST010723-006(T) differed from these closest relatives by 9-19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006(T) constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006(T) (=NRRL B-41100(T)=JCM 12884(T)).
从香港海域的附着海绵（Mycale adhaerens）表面分离出一株革兰氏阴性、无芽孢、短杆状细菌（UST010723-006(T)）。UST010723-006(T)细胞无鞭毛，不运动。菌落呈浅橙色，直径2-4毫米，凸起，表面光滑，边缘完整半透明。在菌落以及菌落下方和相邻的琼脂基质中均观察到气泡。UST010723-006(T)为异养菌，严格需氧，生长需要NaCl（2.0-6.0%）。它在pH 5.0-10.0以及12至44摄氏度之间生长。基于16S rRNA基因序列的系统发育分析将UST010723-006(T)置于变形菌门γ亚纲的假交替单胞菌属内。DNA G+C含量为40.6 mol%，主要脂肪酸为12:0 3-OH、14:0、15:0 iso 2-OH、16:0、16:1ω7、17:1ω8和18:1ω7（总共占总量的75.9%）。这些数据支持UST010723-006(T)属于假交替单胞菌属。其最接近的亲缘种是浅紫假交替单胞菌（Pseudoalteromonas luteviolacea）、酚假交替单胞菌（P. phenolica）、红假交替单胞菌（P. rubra）和鲁氏假交替单胞菌（P. ruthenica），相似性值在95.4%至96.8%之间。UST010723-006(T)与这些最接近的亲缘种在9至19个特征上存在差异。分子证据以及表型特征表明，UST010723-006(T)构成假交替单胞菌属内的一个新物种。为此细菌提议命名为海绵假交替单胞菌（Pseudoalteromonas spongiae sp. nov.）。模式菌株为UST010723-006(T)（=NRRL B-41100(T)=JCM 12884(T)）。
