Okada Mika, Kawano Kimiko, Kura Fumiaki, Amemura-Maekawa Junko, Watanabe Haruo, Yagita Kenji, Endo Takuro, Suzuki Sen
Miyazaki Prefectural Institute for Public Health and Environment.
Kansenshogaku Zasshi. 2005 Jun;79(6):365-74. doi: 10.11150/kansenshogakuzasshi1970.79.365.
In July 2002, a large outbreak of legionellosis occurred in a bathhouse with spa facilities in Miyazaki Prefecture. Two hundred-ninety-five patients (including suspected cases) that had pneumonia and/or symptoms of fever, cough and so forth were reported; 37% of them were hospitalized and seven people died. In environmental investigations, Legionella pneumophila serogroups (SGs) land 8, L. dumoffii, L. londiniensis, some other Legionella species and many kinds of amoeba were isolated from 55 samples of bathtub water, tank water, filters and so forth in the spa facilities. The dominant isolates from the bathtab waters belonged to L. londiniensis, L. dumoffii and L. pneumophila SG1, and their maximum concentrations were 1.5 x 10(6), 5.2 x 10(5) and 1.6 x 10(5) cfu/100 mL, respectively. L. pneumophila SG1 strains isolated from bathtub water, tank water, filters and sputa of patients showed a indistinguishable DNA fingerprint pattern by pulsed-field gel electrophoresis (PFGE), confirming that the source of infection was the spa water. Our study indicate that spas may be a significant health hazard if hygienic management fails.
2002年7月，宫崎县一家设有温泉设施的澡堂发生了大规模军团病疫情。报告了295名患有肺炎和/或发热、咳嗽等症状的患者（包括疑似病例）；其中37%住院治疗，7人死亡。在环境调查中，从温泉设施的55份浴缸水、水箱水、过滤器等样本中分离出嗜肺军团菌血清群（SGs）1型和8型、杜莫夫军团菌、伦敦军团菌、其他一些军团菌属物种以及多种阿米巴。从浴缸水中分离出的主要菌株属于伦敦军团菌、杜莫夫军团菌和嗜肺军团菌SG1型，其最大浓度分别为1.5×10⁶、5.2×10⁵和1.6×10⁵ cfu/100 mL。通过脉冲场凝胶电泳（PFGE），从浴缸水、水箱水、过滤器和患者痰液中分离出的嗜肺军团菌SG1菌株显示出无法区分的DNA指纹图谱，证实感染源是温泉水。我们的研究表明，如果卫生管理不善，温泉可能会对健康造成重大危害。
