Yang Jing, Zhan Xiang-hong, Sun Ye
Changchun College of Traditional Chinese Medicine, Changchun.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Jun;25(6):531-3.
To investigate the effect of Sijunzi decoction (SJZD) on malondialdehyde (MDA) content and telomerase activity in heart, liver and brain tissues of D-galactose (D-gal) induced aging model mice.
D-gal aging mice model was established by cervicodorsal region subcutaneous injection with 10% D-gal once a day for six successive months. The model mice in the low-, middle- and high-dose SJZD treated groups were treated with SJZD in a dose of 6 g/kg, 12 g/kg, 24 g/kg per day respectively in the volume of 0.2 ml/10 g for 6 successive weeks. While the mice in the normal control group (NCG, non-modeled) and the model control group (MCG, modeled but untreated) were treated with distilled water instead. The MDA content and telomerase activity in heart, liver and brain tissues of mice was measured with TBA colorimetric method and PCR-ELISA respectively.
In MCG, the MDA content in heart, liver and brain tissues was significantly higher (P < 0.01), and the telomerase activity in liver and heart tissues was significantly lower (P < 0.01) but that in brain tissue was insignificant different to that in NCG (P > 0.05) respectively. As compared with MCG, the MDA content was significantly lower in the three SJZD treated group (P < 0.01). In comparison of telomerase activity between MCG and SJZD treated groups, it was shown that in heart tissue, there was an increased trend of the activity in the low-dose and middle-dose group, but with statistical insignificance (P > 0.05), but it did show a significant increase in the high-dose group (P < 0.05); in liver tissue no significant difference was shown between the three SJZD treated groups and MCG (P > 0.05); as for that in brain tissue, significant increase only shown in the high-dose group (P < 0.01).
SJZD can antagonize free radical injury, decrease the MDA content of heart, liver and brain in D-gal aging mice, and increase the telomerase activity in heart and brain tissues but with no effect on that in liver tissue.
探讨四君子汤对D-半乳糖（D-gal）诱导的衰老模型小鼠心、肝、脑组织中丙二醛（MDA）含量及端粒酶活性的影响。
通过颈背部皮下注射10% D-半乳糖，每日1次，连续6个月建立D-gal衰老小鼠模型。低、中、高剂量四君子汤治疗组的模型小鼠分别以6 g/kg、12 g/kg、24 g/kg的剂量，按0.2 ml/10 g的体积每日灌胃给予四君子汤，连续6周。正常对照组（NCG，未造模）和模型对照组（MCG，造模但未治疗）小鼠则给予蒸馏水灌胃。分别采用硫代巴比妥酸（TBA）比色法和聚合酶链反应-酶联免疫吸附测定法（PCR-ELISA）检测小鼠心、肝、脑组织中MDA含量及端粒酶活性。
与NCG相比，MCG小鼠心、肝、脑组织中MDA含量显著升高（P < 0.01），肝和心脏组织中端粒酶活性显著降低（P < 0.01），而脑组织中端粒酶活性与NCG相比差异无统计学意义（P > 0.05）。与MCG相比，四君子汤三个治疗组小鼠的MDA含量均显著降低（P < 0.01）。比较MCG与四君子汤治疗组的端粒酶活性，结果显示：在心脏组织中，低剂量和中剂量组端粒酶活性有升高趋势，但差异无统计学意义（P > 0.05），高剂量组端粒酶活性显著升高（P < 0.05）；在肝脏组织中，四君子汤三个治疗组与MCG相比差异无统计学意义（P > 0.05）；在脑组织中，仅高剂量组端粒酶活性显著升高（P < 0.01）。
四君子汤可拮抗自由基损伤，降低D-gal衰老小鼠心、肝、脑的MDA含量，提高心、脑组织中端粒酶活性，但对肝脏组织中端粒酶活性无影响。
