转化生长因子β对小鼠髌骨解剖结构完整的关节软骨中蛋白聚糖合成的抑制作用。
Inhibition of proteoglycan synthesis by transforming growth factor beta in anatomically intact articular cartilage of murine patellae.
作者信息
van der Kraan P M, Vitters E L, van den Berg W B
机构信息
Department of Rheumatology, University Hospital Nijmegen, The Netherlands.
出版信息
Ann Rheum Dis. 1992 May;51(5):643-7. doi: 10.1136/ard.51.5.643.
Abstract
The effect of transforming growth factor beta (TGF beta) on proteoglycan synthesis and degradation in anatomically intact articular cartilage of murine patellae was studied. Exogenously added TGF beta up to a concentration of 200 pmol/l had no effect on proteoglycan synthesis in intact articular cartilage. Neutralisation of endogenously produced TGF beta with a specific monoclonal antibody to TGF beta, however, led to stimulation of proteoglycan synthesis, indicating that TGF beta itself inhibits proteoglycan synthesis in anatomically intact cartilage. Transforming growth factor beta decreased the degradation of proteoglycans in intact cartilage in the absence of fetal calf serum or insulin-like growth factor 1. In the presence of fetal calf serum or insulin-like growth factor 1, TGF beta had no additional effect on proteoglycan breakdown.
摘要
研究了转化生长因子β（TGF-β）对小鼠髌骨解剖结构完整的关节软骨中蛋白聚糖合成与降解的影响。外源性添加浓度高达200 pmol/l的TGF-β对完整关节软骨中的蛋白聚糖合成没有影响。然而，用针对TGF-β的特异性单克隆抗体中和内源性产生的TGF-β会导致蛋白聚糖合成受到刺激，这表明TGF-β本身在解剖结构完整的软骨中抑制蛋白聚糖合成。在没有胎牛血清或胰岛素样生长因子1的情况下，转化生长因子β降低了完整软骨中蛋白聚糖的降解。在存在胎牛血清或胰岛素样生长因子1的情况下，TGF-β对蛋白聚糖分解没有额外影响。
相似文献
1
Inhibition of proteoglycan synthesis by transforming growth factor beta in anatomically intact articular cartilage of murine patellae.转化生长因子β对小鼠髌骨解剖结构完整的关节软骨中蛋白聚糖合成的抑制作用。
Ann Rheum Dis. 1992 May;51(5):643-7. doi: 10.1136/ard.51.5.643.
2
Protection from interleukin 1 induced destruction of articular cartilage by transforming growth factor beta: studies in anatomically intact cartilage in vitro and in vivo.转化生长因子β对白细胞介素1诱导的关节软骨破坏的保护作用：体外和体内解剖结构完整软骨的研究
Ann Rheum Dis. 1993 Mar;52(3):185-91. doi: 10.1136/ard.52.3.185.
3
Maintenance of the synthesis of large proteoglycans in anatomically intact murine articular cartilage by steroids and insulin-like growth factor I.类固醇和胰岛素样生长因子I对解剖结构完整的小鼠关节软骨中大型蛋白聚糖合成的维持作用。
Ann Rheum Dis. 1993 Oct;52(10):734-41. doi: 10.1136/ard.52.10.734.
4
5
Transforming growth factor-beta and insulin-like growth factor-1 restore proteoglycan metabolism of bovine articular cartilage after depletion by retinoic acid.转化生长因子-β和胰岛素样生长因子-1可恢复经视黄酸耗竭后的牛关节软骨蛋白聚糖代谢。
Arch Biochem Biophys. 1994 Nov 15;315(1):190-8. doi: 10.1006/abbi.1994.1489.
6
The recovery of articular cartilage in explant culture from interleukin-1 alpha: effects on proteoglycan synthesis and degradation.白细胞介素-1α对关节软骨外植体培养物的恢复作用：对蛋白聚糖合成与降解的影响
Matrix Biol. 1994 Apr;14(3):263-71. doi: 10.1016/0945-053x(94)90190-2.
7
In vivo protection against interleukin-1-induced articular cartilage damage by transforming growth factor-beta 1: age-related differences.转化生长因子-β1对白细胞介素-1诱导的关节软骨损伤的体内保护作用：年龄相关差异
Ann Rheum Dis. 1994 Sep;53(9):593-600. doi: 10.1136/ard.53.9.593.
8
Transforming growth factor-beta stimulates retinoic acid-induced proteoglycan depletion in intact articular cartilage.转化生长因子-β刺激全层关节软骨中视黄酸诱导的蛋白聚糖消耗。
Arch Biochem Biophys. 1994 Sep;313(2):241-7. doi: 10.1006/abbi.1994.1383.
9
10
The role and content of endogenous insulin-like growth factor-binding proteins in bovine articular cartilage.内源性胰岛素样生长因子结合蛋白在牛关节软骨中的作用及含量
Arch Biochem Biophys. 1997 Jul 15;343(2):164-72. doi: 10.1006/abbi.1997.0166.
引用本文的文献
1
Growth factors that drive aggrecan synthesis in healthy articular cartilage. Role for transforming growth factor-β?驱动健康关节软骨中聚集蛋白聚糖合成的生长因子。转化生长因子-β的作用？
Osteoarthr Cartil Open. 2024 Mar 7;6(2):100459. doi: 10.1016/j.ocarto.2024.100459. eCollection 2024 Jun.
2
TGFβ/BMP Signaling Pathway in Cartilage Homeostasis.TGFβ/BMP 信号通路在软骨稳态中的作用。
Cells. 2019 Aug 24;8(9):969. doi: 10.3390/cells8090969.
3
Evaluating Osteoarthritic Chondrocytes through a Novel 3-Dimensional In Vitro System for Cartilage Tissue Engineering and Regeneration.通过用于软骨组织工程和再生的新型三维体外系统评估骨关节炎软骨细胞。
Cartilage. 2012 Apr;3(2):128-40. doi: 10.1177/1947603511429698.
4
A novel in vitro bovine cartilage punch model for assessing the regeneration of focal cartilage defects with biocompatible bacterial nanocellulose.一种用于评估生物相容性细菌纳米纤维素修复局灶性软骨缺损再生情况的新型体外牛软骨打孔模型。
Arthritis Res Ther. 2013;15(3):R59. doi: 10.1186/ar4231.
5
Growth factor regulation of growth factors in articular chondrocytes.关节软骨细胞中生长因子对生长因子的调控
J Biol Chem. 2009 Mar 13;284(11):6697-704. doi: 10.1074/jbc.M807859200. Epub 2009 Jan 9.
6
Differential effects of interleukin-1 and transforming growth factor beta on the synthesis of small proteoglycans by rabbit articular chondrocytes cultured in alginate beads as compared to monolayers.与单层培养相比，白细胞介素-1和转化生长因子β对藻酸盐珠中培养的兔关节软骨细胞合成小蛋白聚糖的不同影响。
Mol Cell Biochem. 1999 Sep;199(1-2):69-80. doi: 10.1023/a:1006947015094.
7
Articular cartilage destruction in experimental inflammatory arthritis: insulin-like growth factor-1 regulation of proteoglycan metabolism in chondrocytes.实验性炎性关节炎中的关节软骨破坏：胰岛素样生长因子-1对软骨细胞蛋白聚糖代谢的调节
Histochem J. 1996 Dec;28(12):835-57. doi: 10.1007/BF02331388.
8
Effect of growth factors on matrix synthesis by human nasal chondrocytes cultured in monolayer and in agar.生长因子对单层培养及琼脂培养的人鼻软骨细胞基质合成的影响。
Eur Arch Otorhinolaryngol. 1996;253(6):336-40. doi: 10.1007/BF00178288.
9
Protection from interleukin 1 induced destruction of articular cartilage by transforming growth factor beta: studies in anatomically intact cartilage in vitro and in vivo.转化生长因子β对白细胞介素1诱导的关节软骨破坏的保护作用：体外和体内解剖结构完整软骨的研究
Ann Rheum Dis. 1993 Mar;52(3):185-91. doi: 10.1136/ard.52.3.185.
10
Maintenance of the synthesis of large proteoglycans in anatomically intact murine articular cartilage by steroids and insulin-like growth factor I.类固醇和胰岛素样生长因子I对解剖结构完整的小鼠关节软骨中大型蛋白聚糖合成的维持作用。
Ann Rheum Dis. 1993 Oct;52(10):734-41. doi: 10.1136/ard.52.10.734.
本文引用的文献
1
Determination of small quantities of sulfate (0-12 nmol) in serum, urine, and cartilage of the mouse.
Anal Biochem. 1987 Jun;163(2):408-17. doi: 10.1016/0003-2697(87)90242-9.
2
Interleukin 1 suppresses expression of cartilage-specific types II and IX collagens and increases types I and III collagens in human chondrocytes.白细胞介素1抑制人软骨细胞中软骨特异性II型和IX型胶原蛋白的表达，并增加I型和III型胶原蛋白的表达。
J Clin Invest. 1988 Dec;82(6):2026-37. doi: 10.1172/JCI113823.
3
Transforming growth factor beta regulates the metabolism of proteoglycans in bovine cartilage organ cultures.转化生长因子β调节牛软骨器官培养物中蛋白聚糖的代谢。
J Biol Chem. 1988 Sep 15;263(26):12828-31.
4
Transforming growth factor beta stimulates collagen and glycosaminoglycan biosynthesis in cultured rabbit articular chondrocytes.转化生长因子β刺激培养的兔关节软骨细胞中胶原蛋白和糖胺聚糖的生物合成。
FEBS Lett. 1988 Jul 4;234(1):172-6. doi: 10.1016/0014-5793(88)81327-9.
5
Effects of transforming growth factor-beta on matrix synthesis by chick growth plate chondrocytes.转化生长因子-β对鸡生长板软骨细胞基质合成的影响。
Endocrinology. 1988 Jun;122(6):2953-61. doi: 10.1210/endo-122-6-2953.
6
Effect of transforming growth factor beta on cell proliferation and glycosaminoglycan synthesis by rabbit growth-plate chondrocytes in culture.
Biochim Biophys Acta. 1988 Apr 2;969(1):91-9. doi: 10.1016/0167-4889(88)90092-4.
7
Effect of transforming growth factor-beta on DNA synthesis by growth plate chondrocytes: modulation by factors present in serum.
Calcif Tissue Int. 1988 Dec;43(6):352-8. doi: 10.1007/BF02553278.
8
9
Stimulation of cartilage-matrix proteoglycan synthesis by morphologically transformed chondrocytes grown in the presence of fibroblast growth factor and transforming growth factor-beta.
J Cell Physiol. 1989 Feb;138(2):329-37. doi: 10.1002/jcp.1041380216.
10
Active and latent forms of transforming growth factor beta activity in synovial effusions.滑膜积液中转化生长因子β活性的活性形式和潜伏形式。
J Exp Med. 1989 Jan 1;169(1):291-6. doi: 10.1084/jem.169.1.291.
Zotero 插件