Zweit J, Goodall R, Cox M, Babich J W, Potter G A, Sharma H L, Ott R J
Joint Department of Physics, Royal Marsden Hospital, Sutton, Surrey, UK.
Eur J Nucl Med. 1992;19(6):418-25. doi: 10.1007/BF00177368.
Clinical utilisation of positron emission tomography could be enhanced by the availability of short-lived radionuclides derived from generator systems. The zinc-62/copper-62 combination is one such system which could be used as a source for a number of copper-62 radiopharmaceuticals. We have developed and optimised a high activity (5.6 GBq, 150 mCi) zinc-62/copper-62 generator to provide 62Cu in a form that is suitable for direct labelling of pyruvaldehyde-bis-(N4-methylthiosemicarbazone)-copper(II), Cu(PTSM). The distribution coefficients of Zn(II) and Cu(II) between anion-exchange resin and various hydrochloric acid/organic solvent mixtures were measured. Based on these measurements a generator eluent of 0.3 M HCl/40% ethanol provided 62Cu in greater than 90% yield in a 3-ml volume. A very low 62Zn breakthrough of less than 3 x 10(-7)% was achieved. Copper-PTSM was successfully labelled with the no-carrier-added 62Cu eluent directly from the generator with 94% radiochemical yield.
放射性核素发生器系统产生的短寿命放射性核素可提高正电子发射断层扫描的临床应用价值。锌-62/铜-62组合就是这样一种系统，可作为多种铜-62放射性药物的来源。我们已开发并优化了一种高活度（5.6 GBq，150 mCi）的锌-62/铜-62发生器，以提供适合直接标记丙酮醛双（N4-甲基硫代半卡巴腙）-铜（II）（Cu(PTSM)）的铜-62。测量了锌（II）和铜（II）在阴离子交换树脂与各种盐酸/有机溶剂混合物之间的分配系数。基于这些测量结果，0.3 M HCl/40%乙醇的发生器洗脱液在3 ml体积中以大于90%的产率提供铜-62。实现了低于3×10(-7)%的极低锌-62突破。无载体添加的铜-62洗脱液直接从发生器成功标记了Cu-PTSM，放射化学产率为94%。
