Fukumura Toshimitsu, Okada Kazuhiro, Suzuki Hisashi, Nakao Ryuji, Mukai Kensaku, Szelecsényi Ferenc, Kovács Zoltán, Suzuki Kazutoshi
Department of Medical Imaging, Radiopharmaceutical Chemistry Section, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Nucl Med Biol. 2006 Aug;33(6):821-7. doi: 10.1016/j.nucmedbio.2006.05.003. Epub 2006 Jul 21.
The goal of this study was to develop an improved (62)Zn/(62)Cu generator based on cation exchange resin and remote preparation at high radioactivity scale for clinical use.
A natural Cu target was irradiated with proton beam in the energy range of 30-->19 MeV at a beam current of 10 muA for 1 h to obtain around 1.7 GBq of (62)Zn. The (62)Zn was isolated from the Cu target on an anion exchange column with more than 97% yield within 2.5 h from the EOB. The (62)Zn/(62)Cu generator was prepared by loading the (62)Zn(2+) on a Sep-Pak plus CM cartridge.
The generator showed high elution efficiency ( approximately 96%) using a small volume (ca. 3 ml) of a 200-mM glycine solution with a very low breakthrough of (62)Zn (<0.1%).
This (62)Zn/(62)Cu generator has been proven to be highly useful as a source of (62)Cu for the synthesis of (62)Cu-labeled compounds. The clinical application of [(62)Cu]Cu-ATSM produced with this generator has been already approved by the Institutional Review Board at the National Institute of Radiological Sciences.
本研究的目标是开发一种基于阳离子交换树脂的改进型（62）锌/（62）铜发生器，并在高放射性规模下进行远程制备以供临床使用。
用能量范围为30→19 MeV、束流为10 μA的质子束照射天然铜靶1小时，以获得约1.7 GBq的（62）锌。从EOB起2.5小时内，在阴离子交换柱上从铜靶中分离出（62）锌，产率超过97%。通过将（62）锌离子加载到Sep-Pak plus CM柱上制备（62）锌/（62）铜发生器。
使用小体积（约3 ml）的200 mM甘氨酸溶液时发生器显示出高洗脱效率（约96%），（62）锌的穿透率非常低（<0.1%）。
这种（62）锌/（62）铜发生器已被证明作为合成（62）铜标记化合物的（62）铜源非常有用。用该发生器生产的[（62）铜]铜-ATSM的临床应用已获国立放射科学研究所机构审查委员会批准。
