Yi Chengla, Li Xinzhi, Xu Weiguo, Chen Anmin
Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(4):445-7. doi: 10.1007/BF02828219.
To compare the expression level of metastasis associated-1 (MTA1) gene in high and low metastatic human osteosarcoma cell lines and examine the relationship of MTA1 expression and the metastasis potentiality of osteosarcoma cells, the expression of MTA1 in MG-63 osteosarcoma cell lines with high and low metastasis potential was detected by semiquantitative TR-PCR. Boyden chamber invasion assay was used to evaluate the invasive capacity in vitro in two osteosarcoma cell lines. The low metastasis MG-63 cells were transfected with MTA1 full-length cDNA expression plasmid by lipofectamine and the changes of MTA1 expression and in vitro invasion potential were examined after the transfection. Our results showed that MG63 cell line with high metastasis potential expressed significantly higher MTA1 than that of MG63 cells with low metastasis as reavealed by RT-PCR. The invasion potential of low metastasis MG63 cell line was increased after MTA1 gene transfection. It is concluded that there may be a relationship between MTA 1 and invasive potentiality of human osteosarcoma cells, and the mechanism of MTA1 in osteosarcoma metastasis and its possible role in associated gene therapy deserve further study.
为比较转移相关基因-1（MTA1）在高转移和低转移人骨肉瘤细胞系中的表达水平，并探讨MTA1表达与骨肉瘤细胞转移潜能的关系，采用半定量TR-PCR检测高转移潜能和低转移潜能的MG-63骨肉瘤细胞系中MTA1的表达。采用Boyden小室侵袭实验评估两种骨肉瘤细胞系的体外侵袭能力。用脂质体将MTA1全长cDNA表达质粒转染低转移的MG-63细胞，转染后检测MTA1表达及体外侵袭潜能的变化。结果显示，RT-PCR结果表明，高转移潜能的MG63细胞系中MTA1的表达明显高于低转移潜能的MG63细胞。MTA1基因转染后，低转移MG63细胞系的侵袭潜能增加。结论：MTA1与人骨肉瘤细胞的侵袭潜能可能存在关联，MTA1在骨肉瘤转移中的机制及其在相关基因治疗中的可能作用值得进一步研究。
