莱姆病神经伯氏疏螺旋体病的聚合酶链反应：一项前瞻性研究。
PCR in lyme neuroborreliosis: a prospective study.
作者信息
Pícha D, Moravcová L, Zdárský E, Maresová V, Hulínský V
机构信息
First Clinic for Infectious Diseases, Second Medical Faculty of Charles University, Faculty Hospital Bulovka, Czech Republic.
出版信息
Acta Neurol Scand. 2005 Nov;112(5):287-92. doi: 10.1111/j.1600-0404.2005.00482.x.
OBJECTIVES
DNA proof is the only widely available direct diagnostic tool in Lyme borreliosis. Sensitive PCR detecting of spirochetal DNA was prepared and a prospective study in neuroborreliosis was performed.
MATERIALS AND METHODS
57 hospitalised patients with active neuroborreliosis and proved CSF antibodies synthesis were examined. Nested-PCR (utilizing three targets) was used for the detection of specific DNA in plasma, CSF and urine.
RESULTS
Before treatment 36 positive patients (63.1%) were found in all tested specimens in parallel, 28 patients (49.1%) were positive in urine, 20 in CSF (35.0%) and 16 in plasma 28.0%). Later only urine was tested and the following results were obtained: 17 positive patients (30.0%) immediately after treatment, 8 (14.0%) after 3 months and one patient persisted positivity after 6 months.
CONCLUSIONS
The highest sensitivity of PCR was achieved in the acute period of neuroborreliosis - 63.1% in three body fluids comparing with CSF antibody synthesis.
目的
DNA检测是莱姆病唯一广泛可用的直接诊断工具。制备了用于检测螺旋体DNA的灵敏PCR，并对神经型莱姆病进行了前瞻性研究。
材料与方法
对57例住院的活动性神经型莱姆病患者且脑脊液抗体合成得到证实的患者进行检查。采用巢式PCR（利用三个靶点）检测血浆、脑脊液和尿液中的特异性DNA。
结果
治疗前，在所有检测标本中同时发现36例阳性患者（63.1%），尿液中28例阳性（49.1%），脑脊液中20例阳性（35.0%），血浆中16例阳性（28.0%）。之后仅检测尿液，结果如下：治疗后立即有17例阳性患者（30.0%），3个月后8例（14.0%），6个月后有1例患者持续阳性。
结论
与脑脊液抗体合成相比，神经型莱姆病急性期PCR的敏感性最高——在三种体液中为63.1%。
Zotero 插件