工业用嗜碱芽孢杆菌菌株的特性描述：五个新物种的提议。
Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species.
作者信息
Nogi Yuichi, Takami Hideto, Horikoshi Koki
机构信息
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
出版信息
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2309-2315. doi: 10.1099/ijs.0.63649-0.
Twenty alkaliphilic bacterial strains from industrial applications or enzyme studies were subjected to a polyphasic taxonomic investigation, including 16S rRNA gene sequencing, determination of genomic DNA G+C content, DNA-DNA hybridization, fatty acid analysis and standard bacteriological characterization. By comparing the groupings obtained based on the genomic DNA G+C content and the construction of a phylogenetic tree based on the 16S rRNA gene sequence, 12 clusters of similar strains were recognized. DNA-DNA hybridization revealed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus: Bacillus wakoensis sp. nov. (type strain N-1T=JCM 9140T=DSM 2521T), Bacillus hemicellulosilyticus sp. nov. (type strain C-11T=JCM 9152T=DSM 16731T), Bacillus cellulosilyticus sp. nov. (type strain N-4T=JCM 9156T=DSM 2522T), Bacillus akibai sp. nov. (type strain 1139T=JCM 9157T=ATCC 43226T) and Bacillus mannanilyticus sp. nov. (type strain AM-001T=JCM 10596T=DSM 16130T).
对来自工业应用或酶学研究的20株嗜碱细菌菌株进行了多相分类学研究，包括16S rRNA基因测序、基因组DNA G+C含量测定、DNA-DNA杂交、脂肪酸分析和标准细菌学特征分析。通过比较基于基因组DNA G+C含量获得的分组以及基于16S rRNA基因序列构建的系统发育树，识别出12个相似菌株簇。DNA-DNA杂交表明，这些簇代表了5个新的基因种。进一步分析支持了芽孢杆菌属中5个新物种的提议：若松芽孢杆菌（Bacillus wakoensis）新种（模式菌株N-1T=JCM 9140T=DSM 2521T）、半纤维素分解芽孢杆菌（Bacillus hemicellulosilyticus）新种（模式菌株C-11T=JCM 9152T=DSM 16731T）、纤维素分解芽孢杆菌（Bacillus cellulosilyticus）新种（模式菌株N-4T=JCM 9156T=DSM 2522T）、秋伯芽孢杆菌（Bacillus akibai）新种（模式菌株1139T=JCM 9157T=ATCC 43226T）和甘露聚糖分解芽孢杆菌（Bacillus mannanilyticus）新种（模式菌株AM-001T=JCM 10596T=DSM 16130T）。
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