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7-脱氮-2'-脱氧腺苷和3-脱氮-2'-脱氧腺苷取代d(A6)序列中的dA：d(A6).d(T6)与B-DNA在3'和5'连接处的差异弯曲

7-Deaza-2'-deoxyadenosine and 3-deaza-2'-deoxyadenosine replacing dA within d(A6)-tracts: differential bending at 3'- and 5'-junctions of d(A6).d(T6) and B-DNA.

作者信息

Seela F, Grein T

机构信息

Laboratorium für Organische und Bioorganische Chemie, Universität Osnabrück, Germany.

出版信息

Nucleic Acids Res. 1992 Jul 11;20(13):2297-306. doi: 10.1093/nar/20.9.2297.

DOI:10.1093/nar/20.9.2297
PMID:1630898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC312345/
Abstract

7-Deaza-2'-deoxyadenosine (1, c7Ad) and 3-deaza-2'-deoxyadenosine (2, c3Ad) have been incorporated into d(AAAAAA) tracts replacing dA at various positions within oligonucleotides. For this purpose suitably protected phosphonates have been prepared and oligonucleotides were synthesized on solid-phase. The oligomers were hybridized with their cognate strands. The duplexes were phosphorylated at OH-5' by polynucleotide kinase and self-ligated to multimers employing T4 DNA ligase. Oligomerized DNA-fragments were analyzed by polyacrylamide gel electrophoresis and the bending was determined from anomalies of electrophoretic mobility. Replacement of dA by c3Ad decreased the bending more than replacement by c7Ad. Reduction of bending was much stronger when the modified nucleosides replaced one or several dA residues at the 3'-site of an d(AAAAAA)-tract whereas replacement at the 5'-site showed no significant influence [1, 2].

摘要

7-脱氮-2'-脱氧腺苷（1，c7Ad）和3-脱氮-2'-脱氧腺苷（2，c3Ad）已被掺入d(AAAAAA)片段中，在寡核苷酸内的不同位置取代dA。为此，制备了适当保护的膦酸酯，并在固相上合成了寡核苷酸。这些寡聚物与其同源链进行杂交。双链体通过多核苷酸激酶在5'-OH处磷酸化，并使用T4 DNA连接酶自连接成多聚体。通过聚丙烯酰胺凝胶电泳分析寡聚化的DNA片段，并根据电泳迁移率的异常情况确定弯曲度。用c3Ad取代dA比用c7Ad取代dA导致的弯曲度降低更多。当修饰的核苷取代d(AAAAAA)片段3'-位点的一个或几个dA残基时，弯曲度的降低要强得多，而在

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81ba/312345/a0e8f1bdb3e1/nar00083-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81ba/312345/a0e8f1bdb3e1/nar00083-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81ba/312345/a0e8f1bdb3e1/nar00083-0099-a.jpg

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本文引用的文献

1
Bent helical structure in kinetoplast DNA.动基体 DNA 的弯曲螺旋结构。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7664-8. doi: 10.1073/pnas.79.24.7664.
2
Sequence-dependent deformational anisotropy of chromatin DNA.染色质DNA的序列依赖性变形各向异性
Nucleic Acids Res. 1980 Sep 11;8(17):4041-53. doi: 10.1093/nar/8.17.4041.
3
Structure of a B-DNA dodecamer. III. Geometry of hydration.B型DNA十二聚体的结构。III. 水合作用的几何学
带有 3-脱氮腺苷和 3-甲基-3-脱氮腺苷取代的 DNA 的热力学特性：在 DNA 小沟中放置疏水性基团的影响。
J Phys Chem B. 2010 Jun 10;114(22):7656-61. doi: 10.1021/jp101004k.
4
pH-Dependent mismatch discrimination of oligonucleotide duplexes containing 2'-deoxytubercidin and 2- or 7-substituted derivatives: protonated base pairs formed between 7-deazapurines and cytosine.含2'-脱氧结核菌素及2-或7-取代衍生物的寡核苷酸双链体的pH依赖性错配识别：7-脱氮嘌呤与胞嘧啶之间形成的质子化碱基对
Nucleic Acids Res. 2006;34(20):5987-6000. doi: 10.1093/nar/gkl719. Epub 2006 Oct 27.
5
Intrinsic curvature in the VP1 gene of SV40: comparison of solution and gel results.猴空泡病毒40（SV40）VP1基因的固有曲率：溶液结果与凝胶结果的比较
Biophys J. 2005 Feb;88(2):1191-206. doi: 10.1529/biophysj.104.039834. Epub 2004 Nov 19.
6
DNA curvature at A tracts containing a non-polar thymine mimic.含非极性胸腺嘧啶类似物的A序列处的DNA弯曲
Nucleic Acids Res. 2003 Feb 1;31(3):1059-66. doi: 10.1093/nar/gkg172.
7
Synthesis and fluorescence study of 7-azaindole in DNA oligonucleotides replacing a purine base.7-氮杂吲哚取代嘌呤碱基在DNA寡核苷酸中的合成与荧光研究。
Spectrochim Acta A Mol Biomol Spectrosc. 2002 Oct;58(12):2595-603. doi: 10.1016/s1386-1425(02)00004-5.
8
Minimising the secondary structure of DNA targets by incorporation of a modified deoxynucleoside: implications for nucleic acid analysis by hybridisation.通过掺入修饰的脱氧核苷使DNA靶标的二级结构最小化：对杂交核酸分析的影响。
Nucleic Acids Res. 2000 Oct 15;28(20):3904-9. doi: 10.1093/nar/28.20.3904.
9
Thermodynamic stability and drug-binding properties of oligodeoxyribonucleotide duplexes containing 3-deazaadenine:thymine base pairs.含3-脱氮腺嘌呤：胸腺嘧啶碱基对的寡脱氧核糖核苷酸双链体的热力学稳定性和药物结合特性。
Nucleic Acids Res. 1993 Apr 25;21(8):1743-6. doi: 10.1093/nar/21.8.1743.
10
Boron-containing oligodeoxyribonucleotide 14mer duplexes: enzymatic synthesis and melting studies.
Nucleic Acids Res. 1995 Nov 11;23(21):4495-501. doi: 10.1093/nar/23.21.4495.
J Mol Biol. 1981 Sep 25;151(3):535-56. doi: 10.1016/0022-2836(81)90009-7.
4
The anatomy of A-, B-, and Z-DNA.A-DNA、B-DNA和Z-DNA的结构
Science. 1982 Apr 30;216(4545):475-85. doi: 10.1126/science.7071593.
5
The pitch of chromatin DNA is reflected in its nucleotide sequence.染色质DNA的螺距反映在其核苷酸序列中。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3816-20. doi: 10.1073/pnas.77.7.3816.
6
Ordered water structure around a B-DNA dodecamer. A quantitative study.B型DNA十二聚体周围的有序水结构。一项定量研究。
J Mol Biol. 1983 Jan 5;163(1):129-46. doi: 10.1016/0022-2836(83)90033-5.
7
Ultrasensitive staining of nucleic acids with silver.核酸的银超敏染色
Anal Biochem. 1982 Nov 1;126(2):374-80. doi: 10.1016/0003-2697(82)90530-9.
8
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.聚丙烯酰胺凝胶中蛋白质的超敏染色显示脑脊液蛋白质存在区域差异。
Science. 1981 Mar 27;211(4489):1437-8. doi: 10.1126/science.6162199.
9
Sequence dependence of the curvature of DNA: a test of the phasing hypothesis.DNA曲率的序列依赖性：相位假说的检验
Biochemistry. 1985 Dec 3;24(25):7033-7. doi: 10.1021/bi00346a001.
10
DNA bending at adenine . thymine tracts.腺嘌呤·胸腺嘧啶序列处的DNA弯曲
Nature. 1986;320(6062):501-6. doi: 10.1038/320501a0.