通过用碘脱氧尿苷预处理细胞，在无辅助病毒的情况下刺激猿猴细胞中的腺病毒复制。
Stimulation of adenovirus replication in simian cells in the absence of a helper virus by pretreatment of the cells with iododeoxyuridine.
作者信息
Jerkofsky M, Rapp F
出版信息
J Virol. 1975 Feb;15(2):253-8. doi: 10.1128/JVI.15.2.253-258.1975.
Abstract
Pretreatment of African green monkey kidney cells with 50 mu g of 5'-iododeoxyruidine (IUdR) per ml can modify their susceptibility to the replication of human adenovirus type 7 in the absence of simian virus 40 (SV40) although this enhancement of adenovirus replication is not as efficient as that of the helper SV40 virus. Since the number of infectious centers remains unchanged after IUdR pretreatment whereas the burst size of virus from each infected cell increases, the IUdR appears to allow each infected cell to produce more virus. Cell DNA synthesis appears to be stimulated in IUdR pretreated cells infected with adenovirus 7, but the host cell DNA synthesized is small enough to remain in the Hirt supernatant fluid. The modification of susceptibility to adenovirus replication and the changed pattern of cell DNA synthesis is stable for at least two additional cell passages of the pretreated cells.
摘要
每毫升含50微克5'-碘脱氧尿苷（IUdR）对非洲绿猴肾细胞进行预处理，可改变其在无猿猴病毒40（SV40）情况下对人7型腺病毒复制的易感性，尽管腺病毒复制的这种增强效果不如辅助性SV40病毒那样显著。由于经IUdR预处理后感染灶数量不变，而每个感染细胞产生的病毒爆发量增加，因此IUdR似乎能使每个感染细胞产生更多病毒。在感染腺病毒7的经IUdR预处理的细胞中，细胞DNA合成似乎受到刺激，但合成的宿主细胞DNA量小到足以留在Hirt上清液中。对腺病毒复制易感性的改变以及细胞DNA合成模式的变化在预处理细胞至少再传两代时仍保持稳定。
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