乳球菌乳亚种热激反应的特性。
Characterization of the Heat Shock Response in Lactococcus lactis subsp. lactis.
机构信息
Department of Food Science, Cornell University, Ithaca, New York 14853.
出版信息
Appl Environ Microbiol. 1991 May;57(5):1408-12. doi: 10.1128/aem.57.5.1408-1412.1991.
The heat shock response in Lactococcus lactis subsp. lactis was characterized with respect to synthesis of a unique set of proteins induced by thermal stress. A shift in temperature from 30 to 42 degrees C was sufficient to arrest the growth of L. lactis subsp. lactis, but growth resumed after a shift back to 30 degrees C. Heat shock at 50 degrees C reduced the viable cell population by 10; however, pretreatment of the cells at 42 degrees C made them more thermoresistant to exposure at 50 degrees C. The enhanced synthesis of approximately 13 proteins was observed in cells labeled with S upon heat shock at 42 degrees C. Of these heat shock-induced proteins, two appeared to be homologs of GroEL and DnaK, based on their molecular weights and reactivity with antiserum against the corresponding Escherichia coli proteins. Therefore, we conclude that L. lactis subsp. lactis displays a heat shock response similar to that observed in other mesophilic bacteria.
乳球菌乳亚种的热激反应的特点是其对热应激诱导的一组独特蛋白质的合成。温度从 30°C 升高到 42°C 足以阻止乳球菌乳亚种的生长，但当温度回到 30°C 时，生长会恢复。在 50°C 下进行热激会使存活细胞数量减少 10%；然而，将细胞在 42°C 下预处理会使它们在 50°C 下暴露时更耐热。在 42°C 下进行热激时，用 S 标记的细胞中观察到大约 13 种蛋白质的合成增加。在这些热激诱导的蛋白质中，有两种似乎是 GroEL 和 DnaK 的同源物，这是基于它们的分子量和与针对相应大肠杆菌蛋白质的抗血清的反应性。因此，我们得出结论，乳球菌乳亚种表现出与其他中温菌相似的热激反应。
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