Wu W M, Bhatnagar L, Zeikus J G
Michigan Biotechnology Institute, Lansing 48909.
Appl Environ Microbiol. 1993 Feb;59(2):389-97. doi: 10.1128/aem.59.2.389-397.1993.
Anaerobic granules degrading pentachlorophenol (PCP) with specific PCP removal activity up to 14.6 mg/g of volatile suspended solids per day were developed in a laboratory-scale anaerobic upflow sludge blanket reactor at 28 degrees C, by using a mixture of acetate, propionate, butyrate, and methanol as the carbon source. The reactor was able to treat synthetic wastewater containing 40 to 60 mg of PCP per liter at a volumetric loading rate of up to 90 mg/liter of reactor volume per day, with a hydraulic retention time of 10.8 to 15 h. PCP removal of more than 99% was achieved. Results of adsorption of PCP by granular biomass indicated that the PCP removal by the granules was due to biodegradation rather than adsorption. A radiotracer assay demonstrated that the PCP-degrading granules mineralized [14C]PCP to 14CH4 and 14CO2. Toxicity test results indicated that syntrophic propionate degraders and acetate-utilizing methanogens were more sensitive to PCP than syntrophic butyrate degraders. The PCP-degrading granules also exhibited a higher tolerance to the inhibition caused by PCP for methane production and degradation of acetate, propionate, and butyrate, compared with anaerobic granules unadapted to PCP.
在28摄氏度的实验室规模厌氧上流污泥床反应器中，通过使用乙酸盐、丙酸盐、丁酸盐和甲醇的混合物作为碳源，培养出了具有高达每天14.6毫克/克挥发性悬浮固体的五氯苯酚（PCP）去除活性的厌氧颗粒。该反应器能够以高达每天90毫克/升反应器体积的容积负荷率处理每升含有40至60毫克PCP的合成废水，水力停留时间为10.8至15小时，PCP去除率超过99%。颗粒生物质对PCP的吸附结果表明，颗粒对PCP的去除是由于生物降解而非吸附。放射性示踪分析表明，降解PCP的颗粒将[14C]PCP矿化为14CH4和14CO2。毒性测试结果表明，与互营丁酸盐降解菌相比，互营丙酸盐降解菌和利用乙酸盐的产甲烷菌对PCP更敏感。与未适应PCP的厌氧颗粒相比，降解PCP的颗粒对PCP对甲烷产生以及乙酸盐、丙酸盐和丁酸盐降解的抑制也表现出更高的耐受性。
