Gentleman S M, Allsop D, Bruton C J, Jagoe R, Polak J M, Roberts G W
Department of Anatomy, St. Mary's Hospital Medical School, London, UK.
Neurosci Lett. 1992 Feb 17;136(1):27-30. doi: 10.1016/0304-3940(92)90639-o.
The distribution of beta-amyloid protein (beta A4) in the frontal and temporal isocortex of 14 Alzheimer's disease brains was examined using a combination of immunohistochemistry and computer image analysis. The area of cortex covered by beta A4 deposits was determined and expressed as a percentage of the total cortical grey matter area in each field of interest. Significantly more beta A4 was found in the grey matter of the sulci as compared to that of the gyral crests in both the frontal and the temporal lobes (P less than 0.05). Furthermore, in each case, greater quantities of beta A4 were observed in the frontal rather than the temporal lobes. This apparent differential vulnerability is likely to reflect underlying anatomical connections or perhaps differences in cell packing density and appears to strengthen the case for an anatomical basis for the spread of the disease pathology.
采用免疫组织化学和计算机图像分析相结合的方法，对14例阿尔茨海默病患者大脑额叶和颞叶等皮质中β-淀粉样蛋白（βA4）的分布进行了研究。测定βA4沉积物覆盖的皮质面积，并表示为每个感兴趣区域皮质灰质总面积的百分比。与额叶和颞叶的脑回嵴灰质相比，在脑沟灰质中发现的βA4明显更多（P<0.05）。此外，在每种情况下，额叶中观察到的βA4量都多于颞叶。这种明显的易损性差异可能反映了潜在的解剖学联系，或者可能是细胞堆积密度的差异，似乎加强了疾病病理扩散的解剖学基础的证据。
