Cobo Fernando, Talavera Paloma, Concha Angel
Stem Cell Bank of Andalucía (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas, 2, 18014 Granada, Spain.
Virology. 2006 Mar 30;347(1):1-10. doi: 10.1016/j.virol.2005.11.026. Epub 2005 Dec 27.
Some stem cell lines may contain an endogenous virus or can be contaminated with exogenous viruses (even of animal origin) and may secrete viral particles or express viral antigens on their surface. Moreover, certain biotechnological products (e.g. bovine fetal serum, murine feeder cells) may contain prion particles. Viral and prion contamination of cell cultures and "feeder" cells, which is a common risk in all biotechnological products derived from the cell lines, is the most challenging and potentially serious outcome to address, due to the difficulty involved in virus and prion detection and the potential to cause serious disease in recipients of these cell products. Stem cell banks should introduce adequate quality assurance programs like the microbiological control program and can provide researchers with valuable support in the standardization and safety of procedures and protocols used for the viral and prion testing and in validation programs to assure the quality and safety of the cells.
一些干细胞系可能含有内源性病毒或受到外源性病毒（甚至是动物源性病毒）污染，可能分泌病毒颗粒或在其表面表达病毒抗原。此外，某些生物技术产品（如牛胎血清、小鼠饲养细胞）可能含有朊病毒颗粒。细胞培养物和“饲养”细胞的病毒和朊病毒污染是所有源自细胞系的生物技术产品中常见的风险，由于病毒和朊病毒检测困难以及这些细胞产品的接受者有感染严重疾病的可能性，这是最具挑战性且可能最严重的问题。干细胞库应引入适当的质量保证计划，如微生物控制计划，并可为研究人员在用于病毒和朊病毒检测的程序和方案的标准化及安全性以及确保细胞质量和安全性的验证计划方面提供宝贵支持。
