阿姆斯特丹散囊菌和黑曲霉将青蒿素微生物转化为5-羟基青蒿素。
Microbial transformation of artemisinin to 5-hydroxyartemisinin by Eurotium amstelodami and Aspergillus niger.
作者信息
Parshikov Igor A, Miriyala Brushapathy, Muraleedharan Kannoth M, Avery Mitchell A, Williamson John S
机构信息
Department of Medicinal Chemistry, University of Mississippi, University, MS 38677, USA.
出版信息
J Ind Microbiol Biotechnol. 2006 May;33(5):349-52. doi: 10.1007/s10295-005-0071-2. Epub 2005 Dec 31.
Transformation of the anti-malarial drug artemisinin by the fungi Eurotium amstelodami and Aspergillus niger were investigated. Cultures were grown in sucrose/malt broth with artemisinin for 14 days and extracted with ethyl acetate. Extracts were characterized by liquid chromatography. Two metabolites from each fungal extract were isolated and identified using mass spectrometry and nuclear magnetic resonance. 5Beta-hydroxyartemisinin and 7beta-hydroxyartemisinin were isolated in 63 and 32% yields, respectively, from the extract of E. amstelodami, and 80 and 19%, respectively, from the extract of A. niger.
研究了真菌阿姆斯特丹散囊菌和黑曲霉对抗疟药物青蒿素的转化作用。将培养物在含有青蒿素的蔗糖/麦芽肉汤中培养14天，并用乙酸乙酯萃取。萃取物通过液相色谱进行表征。使用质谱和核磁共振对每种真菌提取物中的两种代谢物进行了分离和鉴定。从阿姆斯特丹散囊菌提取物中分别以63%和32%的产率分离出5β-羟基青蒿素和7β-羟基青蒿素，从黑曲霉提取物中分别以80%和19%的产率分离出这两种物质。
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