吸入麻醉剂和高压对二棕榈酰-二肉豆蔻酰磷脂酰胆碱混合双层膜相图的拮抗作用。
The antagonistic effect of an inhalation anesthetic and high pressure on the phase diagram of mixed dipalmitoyl-dimyristoylphosphatidylcholine bilayers.
作者信息
Trudell J R, Payan D G, Chin J H, Cohen E N
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):210-3. doi: 10.1073/pnas.72.1.210.
Abstract
Several workers have shown that phase transition-related changes in membrane lipids have a profound effect on membrane-solvated protein function. This phase transition temperature dependence has been explained as resulting from the formation of lateral phase separations within the membrane bilayer. The present study demonstrates that a clinical concentration of an inhalation anesthetic produces changes in both the phase transition temperature of pure lipid bilayers and the lateral phase separation temperature of mixed dipalmitoyl- and dimyristoylphosphatidylcholine bilayers of a magnitude sufficient to influence protein function. It is further shown that pressure is able to antagonize the effect of the anesthetic on these transition temperatures. It is proposed that anesthetic action within nerve membranes may be the result of changes in the lateral phase separation-controlled environment of the membrane-solvated proteins essential to nerve function.
摘要
几位研究人员已经表明，膜脂中与相变相关的变化对膜溶剂化蛋白的功能有深远影响。这种相变温度依赖性被解释为是由膜双层内横向相分离的形成所致。本研究表明，临床浓度的吸入麻醉剂会使纯脂质双层的相变温度以及二棕榈酰和二肉豆蔻酰磷脂酰胆碱混合双层的横向相分离温度发生变化，其变化幅度足以影响蛋白质功能。进一步研究表明，压力能够拮抗麻醉剂对这些转变温度的影响。有人提出，神经膜内的麻醉作用可能是神经功能所必需的膜溶剂化蛋白的横向相分离控制环境发生变化的结果。
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