Ogawa F, Shimizu K, Muroi E, Hara T, Hasegawa M, Takehara K, Sato S
Department of Dermatology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Rheumatology (Oxford). 2006 Jul;45(7):815-8. doi: 10.1093/rheumatology/kel012. Epub 2006 Jan 31.
To determine serum levels and clinical correlation of 8-isoprostane, which is produced in vivo through free radical-catalysed peroxidation of arachidonic acid and reflects oxidative stress, in patients with systemic sclerosis (SSc).
Serum 8-isoprostane levels from 32 patients with diffuse cutaneous SSc (dSSc) and 25 patients with limited cutaneous SSc (lSSc) were examined by enzyme-linked immunosorbent assay.
Serum 8-isoprostane levels were elevated in dSSc and lSSc patients by 75-fold compared with normal controls (n=32). Serum 8-isoprostane levels correlated negatively with pulmonary function, such as percentage vital capacity and diffusion capacity for carbon monoxide, and correlated positively with renal vascular damage determined by colour flow Doppler ultrasonography. Serum 8-isoprostane levels also correlated positively with serum levels of IgG and anti-agalactosyl IgG autoantibody.
Increased 8-isoprostane levels correlated with the severity of pulmonary fibrosis, the extent of renal vascular damage and immunological abnormalities in SSc, suggesting that enhanced oxidative stress is related to the development of SSc.
测定系统性硬化症（SSc）患者体内通过花生四烯酸自由基催化过氧化产生并反映氧化应激的8-异前列腺素的血清水平及其临床相关性。
采用酶联免疫吸附测定法检测32例弥漫性皮肤型SSc（dSSc）患者和25例局限性皮肤型SSc（lSSc）患者的血清8-异前列腺素水平。
dSSc和lSSc患者的血清8-异前列腺素水平较正常对照组（n = 32）升高了75倍。血清8-异前列腺素水平与肺功能呈负相关，如肺活量百分比和一氧化碳弥散量，与彩色多普勒超声测定的肾血管损伤呈正相关。血清8-异前列腺素水平还与IgG及抗半乳糖基IgG自身抗体的血清水平呈正相关。
8-异前列腺素水平升高与SSc患者肺纤维化的严重程度、肾血管损伤程度及免疫异常相关，提示氧化应激增强与SSc发病有关。
