葡萄籽提取物对大鼠中枢神经系统中与年龄相关的氧化性DNA损伤的调节作用。

Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats.

作者信息

Balu Muthaiya, Sangeetha Purushotham, Murali Ganesan, Panneerselvam Chinnakannu

机构信息

Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.

出版信息

Brain Res Bull. 2006 Feb 15;68(6):469-73. doi: 10.1016/j.brainresbull.2005.10.007. Epub 2005 Nov 2.

DOI:10.1016/j.brainresbull.2005.10.007

PMID:16459205

Abstract

Aging is the accumulation of diverse deleterious changes in the cells and tissues leading to increased risk of diseases. Oxidative stress is considered as a major risk factor and contributes to age related increase in DNA oxidation and DNA protein cross-links in central nervous system during aging. In the present study, we have evaluated the salubrious role of grape seed extract on accumulation of oxidative DNA damage products such as 8-OHdG and DNA protein cross-links in aged rats. Male albino rats of Wistar strain were divided into four groups: Group I, young control rats; Group II, young rats treated with grape seed extract (100 mg/kg b.wt.) for 30 days; Group III, aged control rats; Group IV, aged rats supplemented with grape seed extract (100 mg/kg b.wt.) for 30 days. Our results, thus, revealed that grape seed extract has inhibiting effect on the accumulation of age-related oxidative DNA damages in spinal cord and in various brain regions such as cerebral cortex, striatum and hippocampus.

摘要

衰老指细胞和组织中各种有害变化的积累，会增加患病风险。氧化应激被认为是一个主要风险因素，在衰老过程中会导致中枢神经系统中与年龄相关的DNA氧化和DNA-蛋白质交联增加。在本研究中，我们评估了葡萄籽提取物对老年大鼠氧化DNA损伤产物（如8-羟基脱氧鸟苷）积累和DNA-蛋白质交联的有益作用。将雄性Wistar品系白化大鼠分为四组：第一组，年轻对照大鼠；第二组，用葡萄籽提取物（100毫克/千克体重）处理30天的年轻大鼠；第三组，老年对照大鼠；第四组，补充葡萄籽提取物（100毫克/千克体重）30天的老年大鼠。因此，我们的结果表明，葡萄籽提取物对脊髓以及大脑各区域（如大脑皮层、纹状体和海马体）中与年龄相关的氧化DNA损伤的积累具有抑制作用。