在乳糖操纵子转录正调控中存在缺陷的大肠杆菌分解代谢基因激活蛋白突变体。
Escherichia coli catabolite gene activator protein mutants defective in positive control of lac operon transcription.
作者信息
Eschenlauer A C, Reznikoff W S
机构信息
Department of Biochemistry, University of Wisconsin, Madison 53706.
出版信息
J Bacteriol. 1991 Aug;173(16):5024-9. doi: 10.1128/jb.173.16.5024-5029.1991.
Abstract
We isolated three Escherichia coli catabolite gene activator protein mutants that are defective in the positive control of transcription initiation from the lac operon promoter region yet retain negative control of transcription from other promoters. One mutant has a substitution of valine for glutamate at residue 72, which lies in the cyclic AMP binding domain and contacts cyclic AMP. The other two mutants have substitutions of asparagine and cysteine for glycine 162, which lies in a surface-exposed turn of the DNA-binding domain. Surprisingly, although all three mutants can repress the lacP2/P3 promoters through the catabolite gene activator protein target site of lac, none displays strong dominance over the ability of wild-type catabolite gene activator protein to stimulate the lacP1 promoter.
摘要
我们分离出了三个大肠杆菌分解代谢基因激活蛋白突变体，它们在从乳糖操纵子启动子区域起始转录的正调控中存在缺陷，但仍保留对其他启动子转录的负调控。一个突变体在位于环磷酸腺苷（cAMP）结合结构域且与cAMP接触的第72位残基处，谷氨酸被缬氨酸取代。另外两个突变体在位于DNA结合结构域表面暴露的转角处的第162位甘氨酸处，分别被天冬酰胺和半胱氨酸取代。令人惊讶的是，尽管所有这三个突变体都能通过乳糖的分解代谢基因激活蛋白靶位点抑制lacP2/P3启动子，但在刺激lacP1启动子的能力方面，没有一个对野生型分解代谢基因激活蛋白表现出强显性。
相似文献
1
Escherichia coli catabolite gene activator protein mutants defective in positive control of lac operon transcription.在乳糖操纵子转录正调控中存在缺陷的大肠杆菌分解代谢基因激活蛋白突变体。
J Bacteriol. 1991 Aug;173(16):5024-9. doi: 10.1128/jb.173.16.5024-5029.1991.
2
Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). I. Saturation and alanine-scanning mutagenesis.大肠杆菌分解代谢物基因激活蛋白（CAP）激活区域的特性研究。I. 饱和突变和丙氨酸扫描诱变
J Mol Biol. 1994 Nov 4;243(4):595-602. doi: 10.1016/0022-2836(94)90034-5.
3
Mutants of the catabolite activator protein of Escherichia coli that are specifically deficient in the gene-activation function.大肠杆菌分解代谢物激活蛋白的突变体，这些突变体在基因激活功能方面存在特异性缺陷。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8315-9. doi: 10.1073/pnas.84.23.8315.
4
Substitution of 2 base pairs (1 base pair per DNA half-site) within the Escherichia coli lac promoter DNA site for catabolite gene activator protein places the lac promoter in the FNR regulon.在大肠杆菌乳糖启动子DNA位点内，将2个碱基对（每个DNA半位点1个碱基对）替换为分解代谢物基因激活蛋白，可使乳糖启动子进入FNR调控子。
J Biol Chem. 1990 Jul 25;265(21):12400-3.
5
Downstream deletion analysis of the lac promoter.
J Bacteriol. 1991 Aug;173(15):4570-7. doi: 10.1128/jb.173.15.4570-4577.1991.
6
Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). II. Role at Class I and class II CAP-dependent promoters.大肠杆菌分解代谢物基因激活蛋白（CAP）激活区域的特性。II. 在I类和II类CAP依赖性启动子中的作用
J Mol Biol. 1994 Nov 4;243(4):603-10. doi: 10.1016/0022-2836(94)90035-3.
7
CAP, the -45 region, and RNA polymerase: three partners in transcription initiation at lacP1 in Escherichia coli.CAP、-45区域与RNA聚合酶：大肠杆菌中lacP1转录起始的三个伙伴。
J Mol Biol. 1998 Sep 25;282(3):495-504. doi: 10.1006/jmbi.1998.2040.
8
Catabolite gene activator protein activation of lac transcription.分解代谢基因激活蛋白对乳糖转录的激活作用。
J Bacteriol. 1992 Feb;174(3):655-8. doi: 10.1128/jb.174.3.655-658.1992.
9
Analysis of the spacer DNA between the cyclic AMP receptor protein binding site and the lac promoter.对环磷酸腺苷受体蛋白结合位点与乳糖启动子之间间隔DNA的分析。
J Bacteriol. 1996 Apr;178(8):2436-9. doi: 10.1128/jb.178.8.2436-2439.1996.
10
The -45 region of the Escherichia coli lac promoter: CAP-dependent and CAP-independent transcription.大肠杆菌乳糖启动子的 -45 区域：依赖于代谢物基因激活蛋白（CAP）和不依赖于 CAP 的转录
J Bacteriol. 1997 Jan;179(2):423-9. doi: 10.1128/jb.179.2.423-429.1997.
引用本文的文献
1
Restoring Global Gene Regulation through Experimental Evolution Uncovers a NAP (Nucleoid-Associated Protein)-Like Behavior of Crp/Cap.通过实验进化恢复全球基因调控揭示了 Crp/Cap 的 NAP（核小体相关蛋白）样行为。
mBio. 2021 Oct 26;12(5):e0202821. doi: 10.1128/mBio.02028-21.
2
The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences.创伤弧菌 SmcR 转录因子的 DNA 结合域具有柔韧性，可与多种 DNA 序列结合。
Nucleic Acids Res. 2021 Jun 4;49(10):5967-5984. doi: 10.1093/nar/gkab387.
3
Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing.全局转录因子CRP/CAP中的突变：来自实验进化和深度测序的见解
Comput Struct Biotechnol J. 2019 Jun 18;17:730-736. doi: 10.1016/j.csbj.2019.05.009. eCollection 2019.
4
Analysis of activator and repressor functions reveals the requirements for transcriptional control by LuxR, the master regulator of quorum sensing in Vibrio harveyi.激活子和阻遏子功能分析揭示了群体感应的主调控因子 LuxR 对转录调控的要求，LuxR 是 Harvey 弧菌中群体感应的主调控因子。
mBio. 2013 Jul 9;4(4):e00378-13. doi: 10.1128/mBio.00378-13.
5
Influence of catabolite repression and inducer exclusion on the bistable behavior of the lac operon.分解代谢物阻遏和诱导物排除对乳糖操纵子双稳态行为的影响。
Biophys J. 2004 Mar;86(3):1282-92. doi: 10.1016/S0006-3495(04)74202-2.
6
Repression of deoP2 in Escherichia coli by CytR: conversion of a transcription activator into a repressor.CytR对大肠杆菌中deoP2的抑制作用：转录激活因子向阻遏物的转变。
EMBO J. 2001 Oct 1;20(19):5392-9. doi: 10.1093/emboj/20.19.5392.
7
Quorum sensing in Vibrio fischeri: analysis of the LuxR DNA binding region by alanine-scanning mutagenesis.费氏弧菌中的群体感应：通过丙氨酸扫描诱变分析LuxR DNA结合区域
J Bacteriol. 2001 Jan;183(1):382-6. doi: 10.1128/JB.183.1.382-386.2001.
8
DNA bending by AraC: a negative mutant.AraC引起的DNA弯曲：一个负向突变体。
J Bacteriol. 1998 Aug;180(16):4227-32. doi: 10.1128/JB.180.16.4227-4232.1998.
9
Negative regulation of IS2 transposition by the cyclic AMP (cAMP)-cAMP receptor protein complex.环磷酸腺苷（cAMP）-cAMP受体蛋白复合物对IS2转座的负调控
J Bacteriol. 1998 May;180(10):2682-8. doi: 10.1128/JB.180.10.2682-2688.1998.
10
A common role of CRP in transcription activation: CRP acts transiently to stimulate events leading to open complex formation at a diverse set of promoters.CRP在转录激活中的常见作用：CRP短暂发挥作用，刺激一系列启动子处导致开放复合物形成的事件。
EMBO J. 1998 Mar 16;17(6):1759-67. doi: 10.1093/emboj/17.6.1759.
本文引用的文献
1
Radiation-induced base substitution mutagenesis in single-stranded DNA phage M13.辐射诱导单链DNA噬菌体M13中的碱基替换诱变
Nature. 1981 Nov 12;294(5837):180-2. doi: 10.1038/294180a0.
2
Regulation of damage-inducible genes in Escherichia coli.大肠杆菌中损伤诱导基因的调控
J Mol Biol. 1982 Sep 25;160(3):445-57. doi: 10.1016/0022-2836(82)90307-2.
3
Cyclic AMP receptor protein: role in transcription activation.环磷酸腺苷受体蛋白：在转录激活中的作用。
Science. 1984 May 25;224(4651):831-8. doi: 10.1126/science.6372090.
4
The locus of sequence-directed and protein-induced DNA bending.序列导向和蛋白质诱导的DNA弯曲位点。
Nature. 1984;308(5959):509-13. doi: 10.1038/308509a0.
5
Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein.大肠杆菌环磷酸腺苷受体蛋白基因的分子克隆与核苷酸测序
Nucleic Acids Res. 1982 Feb 25;10(4):1345-61. doi: 10.1093/nar/10.4.1345.
6
Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter.CRP-cAMP激活乳糖操纵子转录起始（P1启动子激活）的机制
J Mol Biol. 1984 Dec 25;180(4):881-909. doi: 10.1016/0022-2836(84)90262-6.
7
Sequence distributions associated with DNA curvature are found upstream of strong E. coli promoters.与DNA弯曲相关的序列分布在大肠杆菌强启动子的上游被发现。
Nucleic Acids Res. 1987 Jan 26;15(2):785-96. doi: 10.1093/nar/15.2.785.
8
Electrolyte gradient gels for DNA sequencing.用于DNA测序的电解质梯度凝胶
Biotechniques. 1988 Nov-Dec;6(10):942-4.
9
Mutations in the lac P2 promoter.乳糖操纵子P2启动子中的突变。
J Bacteriol. 1987 May;169(5):1812-7. doi: 10.1128/jb.169.5.1812-1817.1987.
10
Overlapping promoters and their control in Escherichia coli: the gal case.大肠杆菌中的重叠启动子及其调控：半乳糖操纵子实例
Proc Natl Acad Sci U S A. 1986 May;83(9):2807-11. doi: 10.1073/pnas.83.9.2807.
Zotero 插件