Okada K, Ikeyama S, Ohishi K, Suzuki H, Sugimoto M, Numakunai S, Chiba T, Murakami K, Davis W C, Ohshima K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Japan.
Vet Pathol. 1993 Mar;30(2):104-10. doi: 10.1177/030098589303000202.
Delayed-type hypersensitivity responses against bovine leukemia virus (BLV) envelope glycoprotein (gp60) were induced in the skin of sheep vaccinated with recombinant vaccinia virus (RVV) expressing BLV glycoprotein. The lesions were characterized by marked infiltration of lymphocytes, slight migration of neutrophils, eosinophils, and macrophages in the dermis to hypodermis, and partial intercellular edema in the reticular layer. Immunohistochemical analysis with monoclonal antibodies demonstrated that the lymphocytic infiltrates consisted mainly of CD8+ T cells (53.7-55.8% at 48 hours post-challenge of BLV), CD4+ T cells (24.7-26.7%), and B cells (11.5-16.9%). The role of CD4+ and CD8+ T cells in suppressing BLV growth in RVV-vaccinated animals is discussed.
在用表达牛白血病病毒（BLV）糖蛋白的重组痘苗病毒（RVV）接种的绵羊皮肤中，诱导出了针对BLV包膜糖蛋白（gp60）的迟发型超敏反应。病变的特征为淋巴细胞显著浸润、中性粒细胞、嗜酸性粒细胞和巨噬细胞从真皮向皮下组织轻度迁移，以及网状层出现部分细胞间水肿。用单克隆抗体进行的免疫组织化学分析表明，淋巴细胞浸润主要由CD8 + T细胞（BLV攻击后48小时占53.7 - 55.8%）、CD4 + T细胞（24.7 - 26.7%）和B细胞（11.5 - 16.9%）组成。本文讨论了CD4 +和CD8 + T细胞在抑制RVV接种动物中BLV生长方面的作用。
