Izumi Yasuhiro, Tsuduku Satoko, Murakami Kenji, Tsuboi Takamitsu, Konishi Misako, Haritani Makoto, Kamiyoshi Takeshi, Kimura Kumiko, Sentsui Hiroshi
National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2006 Feb;68(2):189-93. doi: 10.1292/jvms.68.189.
An outbreak of contagious mastitis occurred among cattle on a farm, and bovine herpesviruses were isolated from the affected mammary tissues, scabs and abscess discharge of the cattle. A bovine herpesvirus type 4 (BoHV-4)-specific fragment was amplified from the isolates by polymerase chain reaction (PCR). Restriction endonuclease analyses demonstrated that the isolates were related to Movar-like European type BoHV-4. To determine the ratio of BoHV-4 subclinical infection in the cattle, a genomic survey was performed by PCR for cattle that were moved to the animal hygiene service station in Ibaraki prefecture. The BoHV-4 genome was occasionally detected in peripheral blood leukocytes, lymph nodes and nervous tissues. The rate of BoHV-4 subclinical infection was relatively high in the cattle.
某农场的牛群中发生了传染性乳腺炎疫情，从患病牛的乳腺组织、痂皮和脓肿分泌物中分离出了牛疱疹病毒。通过聚合酶链反应（PCR）从分离株中扩增出了牛疱疹病毒4型（BoHV-4）特异性片段。限制性内切酶分析表明，这些分离株与欧洲Movar样型BoHV-4相关。为了确定牛群中BoHV-4亚临床感染的比例，对转移到茨城县动物卫生服务站的牛进行了PCR基因组调查。在外周血白细胞、淋巴结和神经组织中偶尔检测到BoHV-4基因组。牛群中BoHV-4亚临床感染率相对较高。
