Appl Environ Microbiol. 1997 Mar;63(3):1124-30. doi: 10.1128/aem.63.3.1124-1130.1997.
Alvinella pompejana is a polychaetous annelid that inhabits high-temperature environments associated with active deep-sea hydrothermal vents along the East Pacific Rise. A unique and diverse epibiotic microflora with a prominent filamentous morphotype is found associated with the worm's dorsal integument. A previous study established the taxonomic positions of two epsilon proteobacterial phylotypes, 13B and 5A, which dominated a clone library of 16S rRNA genes amplified by PCR from the epibiotic microbial community of an A. pompejana specimen. In the present study deoxyoligonucleotide PCR primers specific for phylotypes 13B and 5A were used to demonstrate that these phylotypes are regular features of the bacterial community associated with A. pompejana. Assaying of other surfaces around colonies of A. pompejana revealed that phylotypes 13B and 5A are not restricted to A. pompejana. Phylotype 13B occurs on the exterior surfaces of other invertebrate genera and rock surfaces, and phylotype 5A occurs on a congener, Alvinella caudata. The 13B and 5A phylotypes were identified and localized on A. pompejana by in situ hybridization, demonstrating that these two phylotypes are, in fact, the prominent filamentous bacteria on the dorsal integument of A. pompejana. These findings indicate that the filamentous bacterial symbionts of A. pompejana are epsilon Proteobacteria which do not have an obligate requirement for A. pompejana.
Alvinella pompejana 是一种多毛环节动物，栖息于东太平洋海隆活跃深海热液喷口附近的高温环境中。与蠕虫背皮相关的是一种独特而多样的附生微生物群，其中有一个突出的丝状形态。先前的研究确定了两个 ε 变形菌的分类位置，即 13B 和 5A，它们在从 A. pompejana 标本的附生微生物群落中通过 PCR 扩增的 16S rRNA 基因克隆文库中占主导地位。在本研究中，使用针对 13B 和 5A 型的脱氧寡核苷酸 PCR 引物，证明这些型是与 A. pompejana 相关的细菌群落的常规特征。对 A. pompejana 菌落周围其他表面的检测表明，13B 和 5A 型不仅限于 A. pompejana。13B 型存在于其他无脊椎动物属和岩石表面的外部表面，而 5A 型存在于同种 Alvinella caudata 中。通过原位杂交对 A. pompejana 进行鉴定和定位，证明这两种型实际上是 A. pompejana 背皮上的突出丝状细菌。这些发现表明，A. pompejana 的丝状细菌共生体是 ε 变形菌，它们不需要与 A. pompejana 共生。
