Zhou Y C, Zheng R L
Department of Biology, Lanzhou University, China.
Biochem Pharmacol. 1991 Aug 22;42(6):1177-9. doi: 10.1016/0006-2952(91)90251-y.
Five phenolic compounds and pyridoxine were studied for their activities as both scavengers of superoxide anions and inhibitors of lipid peroxidation. The superoxide anions were generated in a phenazin methosulfate-NADH system and were assayed by the reduction of nitroblue tetrazolium. The superoxide anion scavenging activities of verbascoside and alizarin yellow R were the strongest, followed by those of caffeic acid and phloridzin; vanillin and pyridoxine exhibited the weakest activity. The concentration values yielding 50% inhibition of lipid peroxidation in mouse liver microsomes were 10(-5) M for verbascoside, 10(-4) M for alizarin yellow R and caffeic acid, and 10(-3) M for phloridzin; vanillin and pyridoxine had almost no antioxidative activity. The inhibition of lipid peroxidation by these individual compounds was much weaker than by butylated hydroxyanisole. The results showed that phenolic compounds and pyridoxine have more than one mechanism of action for free radicals and are able to suppress free radical processes at two stages: the formation of superoxide anions and the production of lipid peroxides.
研究了5种酚类化合物和吡哆醇作为超氧阴离子清除剂和脂质过氧化抑制剂的活性。超氧阴离子在硫酸吩嗪 - 烟酰胺腺嘌呤二核苷酸（NADH）系统中产生，并通过硝基蓝四唑的还原进行测定。毛蕊花糖苷和茜素黄R的超氧阴离子清除活性最强，其次是咖啡酸和根皮苷；香草醛和吡哆醇的活性最弱。在小鼠肝微粒体中产生50%脂质过氧化抑制作用的浓度值，毛蕊花糖苷为10^(-5) M，茜素黄R和咖啡酸为10^(-4) M，根皮苷为10^(-3) M；香草醛和吡哆醇几乎没有抗氧化活性。这些单个化合物对脂质过氧化的抑制作用远弱于丁基羟基茴香醚。结果表明，酚类化合物和吡哆醇对自由基具有多种作用机制，并且能够在两个阶段抑制自由基过程：超氧阴离子的形成和脂质过氧化物的产生。
