Holse Mille, Assing Kristian, Poulsen Lars K
Laboratory for Medical Allergology 7542, National University Hospital, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
Clin Mol Allergy. 2006 Apr 19;4:6. doi: 10.1186/1476-7961-4-6.
Chemokine receptors have been suggested to be preferentially expressed on CD4+ T cells with CCR3 and CCR8 linked to the T helper (Th) 2 subset and CCR5 and CXCR3 to the Th1 subset, however this remains controversial.
Our aim was to compare the CCR3, CCR5, CCR8 and CXCR3 expression in memory Th cells from allergic, asymptomatically sensitized and healthy individuals.
Peripheral blood mononuclear cells from 8 pollen allergic rhinitis patients, 10 asymptomatically sensitized and 10 healthy individuals were stimulated for 7 days with allergen or tetanus toxoid. CCR3, CCR5, CCR8, CXCR3, CD4 and CD45RO were detected by flow cytometry.
No differences in chemokine receptor expression were observed between the three groups on day 0, and seven days of unstimulated culture did not change the expression. Both antigenic stimuli increased the chemokine receptor expression, tetanus toxoid being the most potent. No differences in percentage chemokine receptor positive memory Th cells were observed between the three groups on day 7. Only a change in MFI for CCR5 was significantly different between the three groups after allergen stimulation of the Th cells.
We conclude that even though allergen and antigen induced increased chemokine receptor expression, no differences in profiles were identified in memory Th cells from patient groups with different atopic status.
有人提出趋化因子受体在CD4 + T细胞上优先表达，其中CCR3和CCR8与辅助性T细胞（Th）2亚群相关，CCR5和CXCR3与Th1亚群相关，但这仍存在争议。
我们的目的是比较变应性、无症状致敏和健康个体记忆性Th细胞中CCR3、CCR5、CCR8和CXCR3的表达。
用变应原或破伤风类毒素刺激8例花粉变应性鼻炎患者、10例无症状致敏个体和10例健康个体的外周血单个核细胞7天。通过流式细胞术检测CCR3、CCR5、CCR8、CXCR3、CD4和CD45RO。
第0天，三组之间趋化因子受体表达无差异，未刺激培养7天也未改变表达。两种抗原刺激均增加趋化因子受体表达，破伤风类毒素作用最强。第7天，三组之间趋化因子受体阳性记忆性Th细胞百分比无差异。Th细胞经变应原刺激后，仅CCR5的平均荧光强度在三组之间有显著差异。
我们得出结论，尽管变应原和抗原诱导趋化因子受体表达增加，但在不同特应状态的患者组记忆性Th细胞中未发现表达谱差异。
