爱泼斯坦 - 巴尔病毒转化的人淋巴细胞细胞核中的病毒和细胞DNA合成
Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.
作者信息
Benz W C, Strominger J L
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2413-7. doi: 10.1073/pnas.72.6.2413.
Abstract
A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as 5 hr, and required ATP, deoxynucleoside triphosphates, magnesium, and a calcium chelator. In nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the Epstein-Barr viral genome, synthesis in vitro was predominately viral in early S phase and cellular in late S phase. These and other data suggested that the DNA synthesis observed in vitro was predominately replicative.
摘要
利用去污剂Brij 58处理人淋巴细胞制备的细胞核，开发了一种体外DNA合成系统。培养淋巴细胞的细胞核能够合成DNA长达5小时，并且需要ATP、脱氧核苷三磷酸、镁和一种钙螯合剂。在携带数百份爱泼斯坦-巴尔病毒基因组的部分同步培养淋巴细胞系的细胞核中，体外合成在S期早期主要是病毒DNA，在S期后期是细胞DNA。这些以及其他数据表明，体外观察到的DNA合成主要是复制性的。
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Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells.经辐照的伯基特细胞培养后正常白细胞中的疱疹病毒型病毒与染色体标记物
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