Whetzel Courtney A, Corwin Elizabeth J, Klein Laura Cousino
Department of Biobehavioral Health, 315 East Health and Human Development Building, The Pennsylvania State University, University Park, PA 16802, USA.
Addict Behav. 2007 Jan;32(1):1-8. doi: 10.1016/j.addbeh.2006.03.007. Epub 2006 Apr 27.
Interferon (IFN)-gamma and IL-10 cytokines, measures of Th1 and Th2 immunity, were examined in 20 healthy nonsmokers (12 males, 8 females) and 19 smokers (11 males, 8 females), aged 19-41 years (23.46+/-0.82 years). Nonsmokers came to the laboratory once; smokers came to the laboratory after ad lib smoking and following 24-h smoking abstinence. Salivary cotinine and expired CO confirmed smoking status. Plasma was collected at the end of each lab session and assayed for peripheral IFN-gamma and IL-10 levels. Among smokers, peripheral IFN-gamma, IL-10, or IFN-gamma/IL-10 ratio levels did not change in response to 24-h smoking abstinence. IFN-gamma levels and IFN-gamma/IL-10 ratios were higher among female smokers while smoking and following 24-h abstinence compared to male smokers in both conditions and compared to male and female nonsmokers. There was no sex or smoking status difference in IL-10 levels. Results suggest that cigarette smoking may have at least short-term damaging effects on the body's normal immune balance, particularly for women.
对20名健康非吸烟者（12名男性，8名女性）和19名吸烟者（11名男性，8名女性）进行了检测，他们的年龄在19至41岁之间（平均年龄23.46±0.82岁），检测指标为作为Th1和Th2免疫指标的干扰素（IFN）-γ和白细胞介素-10细胞因子。非吸烟者仅到实验室一次；吸烟者在随意吸烟后以及戒烟24小时后到实验室。唾液可替宁和呼出一氧化碳证实了吸烟状态。在每次实验室检测结束时采集血浆，检测外周血IFN-γ和白细胞介素-10水平。在吸烟者中，外周血IFN-γ、白细胞介素-10或IFN-γ/白细胞介素-10比值水平在戒烟24小时后没有变化。在吸烟时和戒烟24小时后，女性吸烟者的IFN-γ水平和IFN-γ/白细胞介素-10比值均高于男性吸烟者，且高于男性和女性非吸烟者。白细胞介素-10水平在性别或吸烟状态上没有差异。结果表明，吸烟可能至少对身体的正常免疫平衡有短期损害作用，尤其是对女性。
