University of California, Berkeley, California 94720.
Plant Physiol. 1969 Oct;44(10):1428-38. doi: 10.1104/pp.44.10.1428.
Ultracentrifugation of barley aleurone cells results in the stratification of organelles thus allowing for a quantitation of those organelles. Gibberellic acid (GA(3))-stimulated alpha-amylase production in stratified cells is reduced by centrifugation at gravitational forces greater than 40,000g. Forces below 30,000g do not affect GA(3)-stimulated alpha-amylase production although stratification of organelles occurs at these forces. The ability of centrifuged cells to respond maximally to GA(3) by producing alpha-amylase is related to the degree of redistribution of organelles within these cells. Thus, recovery of cells from centrifugation at forces below 30,000g is rapid, while recovery from forces above 40,000g is slow.
对大麦糊粉层细胞进行超速离心会导致细胞器分层，从而可以对这些细胞器进行定量分析。在离心力大于 40,000g 的情况下，分层细胞中赤霉素（GA(3)）刺激的α-淀粉酶的产生会减少。低于 30,000g 的力不会影响 GA(3)刺激的α-淀粉酶的产生，尽管在这些力下细胞器会分层。经离心处理的细胞通过产生α-淀粉酶对 GA(3)产生最大反应的能力与细胞器在这些细胞内重新分布的程度有关。因此，从低于 30,000g 的力下离心回收细胞的速度很快，而从高于 40,000g 的力下离心回收细胞的速度很慢。
