Zamora-Ros Raul, Urpí-Sardà Mireia, Lamuela-Raventós Rosa M, Estruch Ramón, Vázquez-Agell Mónica, Serrano-Martínez Manuel, Jaeger Walter, Andres-Lacueva Cristina
Nutrition and Food Science Department-CeRTA, Pharmacy School, Hospital Clínic, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain.
Clin Chem. 2006 Jul;52(7):1373-80. doi: 10.1373/clinchem.2005.065870. Epub 2006 May 4.
Nutritional biomarkers may be better measures of dietary exposure than self-reported dietary data. We evaluated resveratrol metabolites, potential biomarkers of wine consumption, in humans after moderate consumption of sparkling, white, or red wines.
We performed 2 randomized, crossover trials and a cohort study. In the first study, 10 healthy men consumed 30 g of ethanol/day as sparkling wine or gin for 28 days. In the second trial, 10 healthy women consumed 20 g of ethanol/day as white or red wine for 28 days. We also evaluated 52 participants in a study on the effects of a Mediterranean diet on primary prevention of cardiovascular disease (the PREDIMED Study). We used liquid chromatography-tandem mass spectrometry to analyze urinary total resveratrol metabolites (TRMs) and predictive values and ROC curve analyses to assess the diagnostic accuracy.
We observed significant increases in TRMs [72.4 (95% confidence interval, 48.5-96.2; P = 0.005), 211.5 (166.6-256.3; P = 0.005), and 560.5 nmol/g creatinine (244.9-876.1; P = 0.005)] after consumption of sparkling, white, or red wine, respectively, but no changes after the washout or gin periods. In the cohort study, the reported daily dose of wine consumption correlated directly with TRMs (r = 0.654; P < 0.001). Using a cutoff of 90 nmol/g, we were able to use TRMs to differentiate wine consumers from abstainers with a sensitivity of 72% (60%-84%); and a specificity of 94% (87%-100%).
Resveratrol metabolites in urine may be useful biomarkers of wine intake in epidemiologic and intervention studies.
营养生物标志物可能比自我报告的饮食数据更能准确衡量饮食摄入量。我们在适量饮用起泡酒、白葡萄酒或红葡萄酒后，对人体中白藜芦醇代谢产物（葡萄酒摄入量的潜在生物标志物）进行了评估。
我们进行了两项随机交叉试验和一项队列研究。在第一项研究中，10名健康男性每天饮用30克乙醇的起泡酒或杜松子酒，持续28天。在第二项试验中，10名健康女性每天饮用20克乙醇的白葡萄酒或红葡萄酒，持续28天。我们还在一项关于地中海饮食对心血管疾病一级预防作用的研究（PREDIMED研究）中评估了52名参与者。我们使用液相色谱-串联质谱法分析尿中总白藜芦醇代谢产物（TRMs），并通过预测值和ROC曲线分析来评估诊断准确性。
饮用起泡酒、白葡萄酒或红葡萄酒后，我们观察到TRMs显著增加[分别为72.4（95%置信区间，48.5 - 96.2；P = 0.005）、211.5（166.6 - 256.3；P = 0.005）和560.5 nmol/g肌酐（244.9 - 876.1；P = 0.005）]，但在洗脱期或饮用杜松子酒期间没有变化。在队列研究中，报告的每日葡萄酒摄入量与TRMs直接相关（r = 0.654；P < 0.001）。使用90 nmol/g的临界值，我们能够利用TRMs将葡萄酒饮用者与戒酒者区分开来，灵敏度为72%（60% - 84%），特异性为94%（87% - 100%）。
尿中的白藜芦醇代谢产物可能是流行病学和干预研究中葡萄酒摄入量的有用生物标志物。
