Zamora-Ros Raul, Urpí-Sardà Mireia, Lamuela-Raventós Rosa M, Estruch Ramon, Martínez-González Miguel Angel, Bulló Mònica, Arós Fernando, Cherubini Antonio, Andres-Lacueva Cristina
Nutrition and Food Science Department-XaRTA, INSA, Pharmacy School, University of Barcelona, 08028 Barcelona, Spain.
Free Radic Biol Med. 2009 Jun 15;46(12):1562-6. doi: 10.1016/j.freeradbiomed.2008.12.023. Epub 2009 Jan 10.
Several clinical and epidemiological studies have shown that moderate wine consumption may exert a protective effect against oxidative stress involved in several diseases, such as cardiovascular and neurodegenerative disorders. However, the epidemiological assessment of wine consumption has usually been obtained using self-reported questionnaires containing less reliable information for assessing total intake than nutritional biomarkers. A reliable biomarker for wine consumption is, therefore, needed. To validate urinary resveratrol metabolites (RMs) as a biomarker of wine consumption in a large cohort of free-living subjects, 1000 consecutive subjects entering a substudy of the PREDIMED trial (Prevención con Dieta Mediterránea) were evaluated. Data were collected in a validated semiquantitative food frequency questionnaire. RMs were measured in morning urine by LC-MS/MS. Urinary RM values correlated directly with reported daily amounts of wine consumed (r=0.895; p<0.001). One drink of wine per week can be detected. Using a cut-off of 411.4 nmol/g creatinine, the measurement of urinary RMs could discriminate wine consumers from non-wine consumers with a sensitivity of 93.3% (95% confidence interval (CI) 91.5-94.7%) and a specificity of 92.1% (CI 90.2-93.7%). Urinary RMs fulfill the criteria to be considered as a nutritional biomarker of wine consumption in a large sample of free-living subjects. This biomarker would provide an additional tool for investigating more precisely the relationship between wine consumption and health benefits.
多项临床和流行病学研究表明，适度饮用葡萄酒可能对多种疾病（如心血管疾病和神经退行性疾病）所涉及的氧化应激发挥保护作用。然而，葡萄酒消费的流行病学评估通常是通过自我报告问卷获得的，与营养生物标志物相比，这些问卷在评估总摄入量方面包含的信息可靠性较低。因此，需要一种可靠的葡萄酒消费生物标志物。为了在一大群自由生活的受试者中验证尿中白藜芦醇代谢物（RMs）作为葡萄酒消费的生物标志物，对参加PREDIMED试验（地中海饮食预防）子研究的1000名连续受试者进行了评估。数据通过经过验证的半定量食物频率问卷收集。通过液相色谱-串联质谱法测定晨尿中的RMs。尿中RM值与报告的每日葡萄酒摄入量直接相关（r = 0.895；p < 0.001）。每周饮用一杯葡萄酒都能被检测到。使用411.4 nmol/g肌酐的截断值，尿中RMs的测量能够以93.3%（95%置信区间（CI）91.5 - 94.7%）的灵敏度和92.1%（CI 90.2 - 93.7%）的特异性区分葡萄酒消费者和非葡萄酒消费者。尿中RMs符合在大量自由生活受试者样本中被视为葡萄酒消费营养生物标志物的标准。这种生物标志物将为更精确地研究葡萄酒消费与健康益处之间的关系提供一个额外的工具。
