Zamora-Ros Raul, Rothwell Joseph A, Achaintre David, Ferrari Pietro, Boutron-Ruault Marie-Christine, Mancini Francesca R, Affret Aurelie, Kühn Tilman, Katzke Verena, Boeing Heiner, Küppel Sven, Trichopoulou Antonia, Lagiou Pagona, La Vecchia Carlo, Palli Domenico, Contiero Paolo, Panico Salvatore, Tumino Rosario, Ricceri Fulvio, Noh Hwayoung, Freisling Heinz, Romieu Isabelle, Scalbert Augustin
1Unit of Nutrition and Cancer,Epidemiology Research Program Catalan Institute of Oncology,Bellvitge Biomedical Research Institute (IDIBELL),08908 Barcelona,Spain.
2Section of Nutrition and Metabolism,International Agency for Research on Cancer (IARC-WHO),69372 Lyon,France.
Br J Nutr. 2017 Jun;117(11):1596-1602. doi: 10.1017/S0007114517001465. Epub 2017 Jun 22.
In vitro studies have shown several beneficial properties of resveratrol. Epidemiological evidence is still scarce, probably because of the difficulty in estimating resveratrol exposure accurately. The current study aimed to assess the relationships between acute and habitual dietary resveratrol and wine intake and urinary resveratrol excretion in a European population. A stratified random subsample of 475 men and women from four countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) cross-sectional study, who had provided 24-h urine samples and completed a 24-h dietary recall (24-HDR) on the same day, were included. Acute and habitual dietary data were collected using standardised 24-HDR software and a validated country-specific dietary questionnaire, respectively. Phenol-Explorer was used to estimate the intake of resveratrol and other stilbenes. Urinary resveratrol was analysed using tandem MS. Spearman's correlation coefficients between estimated dietary intakes of resveratrol and other stilbenes and consumption of wine, their main food source, were very high (r>0·9) when measured using dietary questionnaires and were slightly lower with 24-HDR (r>0·8). Partial Spearman's correlations between urinary resveratrol excretion and intake of resveratrol, total stilbenes or wine were found to be higher when using the 24-HDR (R 2 partial approximately 0·6) than when using the dietary questionnaires (R 2 partial approximately 0·5). Moderate to high correlations between dietary resveratrol, total stilbenes and wine, and urinary resveratrol concentrations were observed. These support the earlier findings that 24-h urinary resveratrol is an effective biomarker of both resveratrol and wine intakes. These correlations also support the validity of the estimation of resveratrol intake using the dietary questionnaire and Phenol-Explorer.
体外研究显示了白藜芦醇的多种有益特性。流行病学证据仍然稀少，这可能是由于准确估计白藜芦醇摄入量存在困难。本研究旨在评估欧洲人群中急性和习惯性饮食白藜芦醇及葡萄酒摄入量与尿白藜芦醇排泄之间的关系。纳入了来自四个国家的475名男性和女性的分层随机子样本，他们参与了欧洲癌症与营养前瞻性调查（EPIC）横断面研究，在同一天提供了24小时尿液样本并完成了24小时饮食回顾（24-HDR）。急性和习惯性饮食数据分别使用标准化的24-HDR软件和经过验证的特定国家饮食问卷收集。使用Phenol-Explorer来估计白藜芦醇和其他芪类化合物的摄入量。使用串联质谱分析尿白藜芦醇。当使用饮食问卷测量时，估计的白藜芦醇和其他芪类化合物饮食摄入量与葡萄酒（它们的主要食物来源）消费之间的斯皮尔曼相关系数非常高（r>0·9），而使用24-HDR时略低（r>0·8）。发现使用24-HDR时尿白藜芦醇排泄与白藜芦醇、总芪类化合物或葡萄酒摄入量之间的偏斯皮尔曼相关性（R 2偏约为0·6）高于使用饮食问卷时（R 2偏约为0·5）。观察到饮食白藜芦醇、总芪类化合物和葡萄酒与尿白藜芦醇浓度之间存在中度至高相关性。这些结果支持了早期的发现，即24小时尿白藜芦醇是白藜芦醇和葡萄酒摄入量的有效生物标志物。这些相关性也支持了使用饮食问卷和Phenol-Explorer估计白藜芦醇摄入量的有效性。
