Fiore Francesca, Von Bergwelt-Baildon Michael S, Drebber Uta, Beyer Marc, Popov Alexey, Manzke Oliver, Wickenhauser Claudia, Baldus Stefan E, Schultze Joachim L
Molecular Tumor Biology and Tumor Immunology, Clinic I for Internal Medicine, University of Cologne, Cologne, Germany.
Leuk Lymphoma. 2006 Apr;47(4):613-22. doi: 10.1080/10428190500360971.
Despite the lack of tumor control, infiltration of immune cells has been demonstrated for several malignancies including non-Hodgkin's lymphoma. Since dendritic cells play a pivotal role in the initiation and control of the immune response, the frequency and phenotype of recently described sub-types of dendritic cells in non-Hodgkin's lymphoma were characterized. Myeloid and plasmacytoid dendritic cells were analysed in 55 non-Hodgkin's lymphoma and 33 reactive lymph nodes by flow cytometry and immunohistochemistry. Overall frequency of dendritic cells in reactive lymph nodes was higher than in non-Hodgkin's lymphoma while the pDC/mDCs ratio was comparable. The low frequency of dendritic cells in infiltrated lymph nodes was confirmed by immunohistochemistry; however, no significant difference in the distribution within lymphoid and tumor tissue was detected. For further characterization of the dendritic cells in non-Hodgkin's lymphoma, the expressions of adhesion molecules, costimulatory molecules, chemokine receptors and activation markers were assessed. Interestingly, a significantly decreased expression of CD62L and CCR7, receptors necessary for homing to lymph nodes, was identified in dendritic cells in non-Hodgkin's lymphoma, potentially explaining the lack of these cells. Taken together, dendritic cells are phenotypically altered and reduced in number in NHL, potentially contributing to the loss of tumor control in these patients.
尽管缺乏肿瘤控制，但已证实免疫细胞浸润存在于包括非霍奇金淋巴瘤在内的几种恶性肿瘤中。由于树突状细胞在免疫反应的启动和控制中起关键作用，因此对非霍奇金淋巴瘤中最近描述的树突状细胞亚型的频率和表型进行了表征。通过流式细胞术和免疫组织化学分析了55例非霍奇金淋巴瘤和33例反应性淋巴结中的髓样和浆细胞样树突状细胞。反应性淋巴结中树突状细胞的总体频率高于非霍奇金淋巴瘤，而浆细胞样树突状细胞/髓样树突状细胞的比例相当。免疫组织化学证实浸润淋巴结中树突状细胞的频率较低；然而，在淋巴组织和肿瘤组织中的分布未检测到显著差异。为了进一步表征非霍奇金淋巴瘤中的树突状细胞，评估了粘附分子、共刺激分子、趋化因子受体和激活标志物的表达。有趣的是，在非霍奇金淋巴瘤的树突状细胞中发现归巢至淋巴结所需的受体CD62L和CCR7的表达显著降低，这可能解释了这些细胞的缺乏。综上所述，非霍奇金淋巴瘤中树突状细胞在表型上发生改变且数量减少，这可能导致这些患者肿瘤控制的丧失。
