Ananda Shalini, Marsden Valerie, Vekemans Katrien, Korkmaz Emine, Tsafnat Naomi, Soon Lilian, Jones Allan, Braet Filip
Australian Key Centre for Microscopy and Microanalysis, Electron Microscope Unit, The University of Sydney NSW 2006, Australia.
J Electron Microsc (Tokyo). 2006 Jun;55(3):151-5. doi: 10.1093/jmicro/dfl015. Epub 2006 Jun 14.
In this study, X-ray micro-computed tomography (CT) was used to reconstruct the fine structure macro- and microvasculature in three dimensions in contrast-enhanced rat liver samples. The subsequent application in the experimental CC531s colorectal cancer model was concurrent with results obtained from confocal microscopy in earlier studies. The en bloc stains osmium tetroxide in combination with uranyl acetate provided an excellent contrasting result for hepatic tissue after a trial of several contrasting agents. X-ray micro-CT allowed us to image the large blood vessels together with the branching sinusoids of hepatic tissue in three dimensions. Furthermore, interruption of the microvasculature was noted when rats were injected with CC531s colorectal cancer cells indicating the presence of hepatic metastases.
在本研究中，利用X射线显微计算机断层扫描（CT）对经对比增强的大鼠肝脏样本中的宏观和微观血管系统的精细结构进行三维重建。随后在实验性CC531s结直肠癌模型中的应用与早期研究中共聚焦显微镜获得的结果一致。在对几种造影剂进行试验后，四氧化锇与醋酸铀酰的整体染色为肝组织提供了出色的对比效果。X射线显微CT使我们能够对肝脏组织的大血管和分支血窦进行三维成像。此外，当给大鼠注射CC531s结直肠癌细胞时，注意到微血管系统受到破坏，这表明存在肝转移。
