Müller Daniel, Hagedorn Peter, Brast Sabine, Heusipp Gerhard, Bielaszewska Martina, Friedrich Alexander W, Karch Helge, Schmidt M Alexander
Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Von-Esmarch-Str. 56, D-48149 Münster, Germany.
J Clin Microbiol. 2006 Jul;44(7):2626-9. doi: 10.1128/JCM.00895-06.
Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.
肠致病性大肠杆菌(EPEC)、非典型肠致病性大肠杆菌和产志贺毒素大肠杆菌在毒力因子谱、临床表现和预后方面存在差异,并且需要不同的治疗措施。我们开发并评估了一种强大的多重聚合酶链反应(PCR),以基于与escV、bfpB、stx1和stx2互补的序列来鉴定这些致病菌群。