McCarthy D A, Rampton D S, Liu Y C
School of Biological Sciences, Queen Mary and Westfield College, London, England.
Clin Exp Immunol. 1991 Dec;86(3):489-93. doi: 10.1111/j.1365-2249.1991.tb02958.x.
Morphological evidence of activation in vivo of circulating neutrophils in patients with inflammatory bowel disease (IBD) was sought by quantitative light microscope examination of toluidine blue-stained preparations made from peripheral venous bloods that had been fixed immediately ex vivo. The proportion of spherical (unactivated) circulating neutrophils was reduced in active Crohn's disease (73%; 46-96 (median; range), n = 11) compared with inactive Crohn's (90%; 45-99; n = 18, P less than 0.01) and normal subjects (94%; 44-98; n = 13, P less than 0.05). There tended to be fewer spherical neutrophils in active ulcerative colitis (77%; 13-96; n = 17) than in quiescent colitis (88%; 57-99, n = 13, P less than 0.1) or normal subjects (P less than 0.05). Activated neutrophils occur in the circulating pool of patients with active IBD and can be detected by light microscopy of peripheral venous blood leucocyte preparations.
通过对离体后立即固定的外周静脉血制成的甲苯胺蓝染色制剂进行定量光学显微镜检查，来探寻炎症性肠病（IBD）患者体内循环中性粒细胞激活的形态学证据。与非活动期克罗恩病（90%；45 - 99；n = 18）和正常受试者（94%；44 - 98；n = 13）相比，活动期克罗恩病患者中球形（未激活）循环中性粒细胞的比例降低（73%；46 - 96（中位数；范围），n = 11，P < 0.01）。活动期溃疡性结肠炎患者中球形中性粒细胞的数量（77%；13 - 96；n = 17）往往比静止期结肠炎患者（88%；57 - 99，n = 13，P < 0.1）或正常受试者（P < 0.05）更少。激活的中性粒细胞存在于活动期IBD患者的循环池中，并且可以通过外周静脉血白细胞制剂的光学显微镜检测到。
