Yamano Toshihiko, Tanida Mamoru, Niijima Akira, Maeda Keiko, Okumura Nobuaki, Fukushima Yoichi, Nagai Katsuya
Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamada-Oka, Suita, Osaka 565-0871, Japan.
Life Sci. 2006 Oct 12;79(20):1963-7. doi: 10.1016/j.lfs.2006.06.038. Epub 2006 Jun 29.
Oral administration of Lactobacillus casei reportedly reduces blood glucose concentrations in a non-insulin-dependent diabetic KK-Ay mouse model. In order to determine if other lactobacillus strains affect glucose metabolism, we evaluated the effect of the probiotic strain Lactobacillus johnsonii La1 (LJLa1) strain on glucose metabolism in rats. Oral administration of LJLa1 via drinking water for 2 weeks inhibited the hyperglycemia induced by intracranial injection of 2-deoxy-D-glucose (2DG). We found that the hyperglucagonemic response induced by 2DG was also suppressed by LJLa1. Oral administration of LJLa1 for 2 weeks also reduced the elevation of blood glucose and glucagon levels after an oral glucose load in streptozotocin-diabetic rats. In addition, we recently observed that intraduodenal injection of LJLa1 reduced renal sympathetic nerve activity and enhanced gastric vagal nerve activity, suggesting that LJLa1 might affect glucose metabolism by changing autonomic nerve activity. Therefore, we evaluated the effect of intraduodenal administration of LJLa1 on adrenal sympathetic nerve activity (ASNA) in urethane-anesthetized rats, since the autonomic nervous system, including the adrenal sympathetic nerve, may be implicated in the control of the blood glucose levels. Indeed, we found that ASNA was suppressed by intraduodenal administration of LJLa1, suggesting that LJLa1 might improve glucose tolerance by reducing glucagon secretion via alteration of autonomic nerve activities.
据报道，口服干酪乳杆菌可降低非胰岛素依赖型糖尿病KK-Ay小鼠模型的血糖浓度。为了确定其他乳酸菌菌株是否会影响葡萄糖代谢，我们评估了益生菌约氏乳杆菌La1（LJLa1）菌株对大鼠葡萄糖代谢的影响。通过饮用水口服LJLa1两周可抑制颅内注射2-脱氧-D-葡萄糖（2DG）诱导的高血糖症。我们发现，LJLa1也可抑制2DG诱导的高胰高血糖素血症反应。对链脲佐菌素诱导的糖尿病大鼠口服LJLa1两周，也可降低口服葡萄糖负荷后血糖和胰高血糖素水平的升高。此外，我们最近观察到十二指肠内注射LJLa1可降低肾交感神经活动并增强胃迷走神经活动，这表明LJLa1可能通过改变自主神经活动来影响葡萄糖代谢。因此，我们评估了十二指肠内给予LJLa1对氨基甲酸乙酯麻醉大鼠肾上腺交感神经活动（ASNA）的影响，因为包括肾上腺交感神经在内的自主神经系统可能参与血糖水平的控制。实际上，我们发现十二指肠内给予LJLa1可抑制ASNA，这表明LJLa1可能通过改变自主神经活动减少胰高血糖素分泌来改善葡萄糖耐量。
