Maurício R, Diniz M, Petrovic M, Amaral L, Peres I, Barceló D, Santana F
Dep. de Ciências e Engenharia do Ambiente, Universidade Nova de Lisboa-Faculdade de Ciências e Tecnologia, Quinta da Torre-2825, Monte da Caparica, Portugal.
Environ Monit Assess. 2006 Jul;118(1-3):75-87. doi: 10.1007/s10661-006-0986-8.
Anthropogenic compounds that are able to disrupt the endocrine system of wildlife species are a major cause for concern and have led to a demand for new screening methods. The identification and quantification of endocrine disruptor compounds at wastewater treatment plant is of major interest to assess the endocrine activity of wastewater treatment plant discharges into the environment. This study consists of a preliminary survey of concentrations of previously selected endocrine disruptor compounds, undertaken to establish environmental concentrations and to support a biological program assay exposing freshwater fish to them. Selected endocrine disrupting chemicals (APEs, bisphenol A and 17 beta-estradiol) were measured in samples from a wastewater treatment plant located in Lisbon (Portugal), using recent commercial enzyme-linked immunosorbent assay kits and also LC-MS/MS. The results show that the wastewater treatment plant treatment process is efficient on the removal of target endocrine disruptor compounds. However, environmentally significant concentrations are still present in the treated effluent. The results also show that enzyme-linked immunosorbent assay kit is suitable for routine analysis of the selected compounds. The results are also useful since the wastewater treatment plant is located in a Mediterranean region, which results in an effluent with its own characteristics.
能够干扰野生动物物种内分泌系统的人为化合物是一个主要的担忧原因，并引发了对新筛选方法的需求。在污水处理厂识别和量化内分泌干扰化合物对于评估污水处理厂排放到环境中的内分泌活性至关重要。本研究包括对先前选定的内分泌干扰化合物浓度的初步调查，旨在确定环境浓度并支持一项将淡水鱼暴露于这些化合物的生物程序分析。使用最新的商业酶联免疫吸附测定试剂盒以及液相色谱-串联质谱法，对位于里斯本（葡萄牙）的一家污水处理厂的样本中的选定内分泌干扰化学物质（烷基酚聚氧乙烯醚、双酚A和17β-雌二醇）进行了测量。结果表明，污水处理厂的处理过程在去除目标内分泌干扰化合物方面是有效的。然而，处理后的废水中仍存在具有环境意义的浓度。结果还表明，酶联免疫吸附测定试剂盒适用于对选定化合物的常规分析。这些结果也很有用，因为该污水处理厂位于地中海地区，其排放的废水具有自身特点。
