Departamento de Química Analítica y Análisis Instrumental and Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA-Nanociencia), Campus Cantoblanco, Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Mikrochim Acta. 2020 Feb 19;187(3):180. doi: 10.1007/s00604-020-4137-7.
A rapid fluorometric method is described for the determination of lactate and cholesterol by using ZnO nanowires (ZnO NWs). The assay is based on the detection of the hydrogen peroxide generated during the enzymatic reactions of the oxidation of lactate or cholesterol. Taking advantage of the electrostatic interactions between the enzymes and the ZnO NWs, two bioconjugates were prepared by mixing the nanomaterial and the enzymes, viz. lactate oxidase (LOx) or cholesterol oxidase (ChOx). The enzymatically generated hydrogen peroxide quenches the fluorescence of the ZnO NWs, which have emission peaks at 384 nm and at 520 nm under 330 nm photoexcitation. HO quenches the 520 nm band more strongly. Response is linear up to 1.9 μM lactate concentration, and up to 1.1 μM cholesterol concentration. Relative standard deviation was found to be 5%. The detection limits for lactate and cholesterol are 0.54 and 0.24 μM, respectively. Graphical abstractSchematic representation of fluorescence assay based on ZnO nanowires photoluminiscence for lactate and colesterol detection.
一种快速荧光法用于通过氧化锌纳米线（ZnO NWs）测定乳酸和胆固醇。该测定基于在乳酸或胆固醇氧化的酶促反应期间产生的过氧化氢的检测。利用酶和 ZnO NWs 之间的静电相互作用，通过混合纳米材料和酶制备了两种生物缀合物，即乳酸氧化酶（LOx）或胆固醇氧化酶（ChOx）。酶促产生的过氧化氢猝灭了 ZnO NWs 的荧光，在 330nm 光激发下，ZnO NWs 的发射峰在 384nm 和 520nm 处。HO 更强烈地猝灭 520nm 带。响应在 1.9μM 乳酸浓度和 1.1μM 胆固醇浓度下呈线性。相对标准偏差为 5%。乳酸和胆固醇的检测限分别为 0.54 和 0.24μM。
