酿酒酵母中自发相互有丝分裂交换的选择与分析。
Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae.
作者信息
Barbera Maria A, Petes Thomas D
机构信息
Department of Molecular Genetics and Microbiology, Box 3054, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12819-24. doi: 10.1073/pnas.0605778103. Epub 2006 Aug 14.
Abstract
We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4x10(-5) per division, a rate approximately 25,000-fold lower than the meiotic rate of cross-overs. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.
摘要
我们开发了一种系统，该系统能够筛选出酿酒酵母中自发有丝分裂交换产生的 reciprocal 产物。利用该系统还可以监测许多其他类型的遗传事件，包括染色体丢失。对于位于第五条染色体（CEN5-CAN1）上的一个120 kb的染色体区间，有丝分裂交换率为每分裂4×10⁻⁵ ，该速率比减数分裂交换率低约25000倍。与减数分裂交换中观察到的抑制现象不同，我们发现在第五条染色体着丝粒附近没有有丝分裂交换的抑制现象。用羟基脲处理细胞可使 reciprocal 交换率大幅提高（38倍），羟基脲是一种能减少核苷酸库并减缓DNA复制的药物。
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