Naik Suresh R, Pilgaonkar Vinaya W, Panda Vandana S
Department of Pharmacology and Toxicology, Principal K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgankar Marg, Cuffe Parade, Colaba, Mumbai 400 005, India.
Phytother Res. 2006 Nov;20(11):1013-6. doi: 10.1002/ptr.1976.
Ginkgo biloba from the traditional Chinese system of medicine has been found to possess neurocognitive enhancing effects. The mechanism of action of Ginkgo seems to be related to its antioxidant properties. In the present study, Ginkgo biloba phytosomes were administered to Wistar rats at 50 mg/kg and 100 mg/kg for 7 and 14 days. Chemical hypoxia was induced by administration of sodium nitrite (75 mg/kg) 1 h after the last administration of treatment. Thirty minutes after sodium nitrite administration, the animals were killed and the cerebral cortex, cerebellum, hippocampus and striatum were isolated and homogenized. The supernatants were used for the estimation of the antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. Ginkgo biloba phytosome treatment was found to increase superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities in all the brain regions compared with those treated only with sodium nitrite. The prevention of depletion of the antioxidant enzymes by sodium nitrite in the presence of Ginkgo biloba phytosomes may be correlated to its antioxidant activity.
来自传统中医体系的银杏已被发现具有增强神经认知的作用。银杏的作用机制似乎与其抗氧化特性有关。在本研究中，将银杏叶磷脂复合物以50毫克/千克和100毫克/千克的剂量给予Wistar大鼠，持续7天和14天。在最后一次给药后1小时，通过给予亚硝酸钠（75毫克/千克）诱导化学性缺氧。给予亚硝酸钠30分钟后，处死动物，分离大脑皮层、小脑、海马和纹状体并匀浆。上清液用于评估抗氧化酶超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶。与仅用亚硝酸钠处理的大鼠相比，发现银杏叶磷脂复合物处理可增加所有脑区中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性。在银杏叶磷脂复合物存在的情况下，亚硝酸钠对抗氧化酶消耗的预防作用可能与其抗氧化活性相关。
