Qu Fengli, Yang Minghui, Shen Guoli, Yu Ruqin
Chemistry and Chemical Engineering College, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
Biosens Bioelectron. 2007 Mar 15;22(8):1749-55. doi: 10.1016/j.bios.2006.08.003. Epub 2006 Sep 11.
Platinum nanowires (PtNWs) prepared by electrodeposition method with the help of porous anodic aluminum oxide (AAO) templates have been solubilized in chitosan (CHIT) together with carbon nantubes (CNTs) to form a PtNW-CNT-CHIT organic-inorganic system. The resulting PtNW-CNT-CHIT material brings capabilities for utilizing synergic action of PtNWs and CNTs to facilitate electron-transfer process in electrochemical sensor design. The PtNW-CNT-CHIT film modified electrode offered a significant decrease in the overvoltage for the hydrogen peroxide and showed to be excellent amperometric sensors for hydrogen peroxide at -0.1 V over a wide range of concentrations, and the sensitivity is 260 microAmM-1cm-2. As an application example, by linking glucose oxidase (GOx), an amplified biosensor toward glucose was prepared. The glucose biosensor exhibits a selective determination of glucose at -0.1 V with a linear response range of 5 x 10(-6) to 1.5 x 10(-2)M with a correlation coefficient of 0.997, and response time <10s. The high sensitivity of the glucose biosensor is up to 30 microAmM-1cm-2 and the detection limit was 3 microM. The biosensor displays rapid response and expanded linear response range, and excellent repeatability and stability.
通过电沉积法借助多孔阳极氧化铝（AAO）模板制备的铂纳米线（PtNWs）已与碳纳米管（CNTs）一起溶解在壳聚糖（CHIT）中，形成PtNW - CNT - CHIT有机 - 无机体系。所得的PtNW - CNT - CHIT材料具有利用PtNWs和CNTs的协同作用来促进电化学传感器设计中电子转移过程的能力。PtNW - CNT - CHIT膜修饰电极使过氧化氢的过电压显著降低，并在 - 0.1 V下对宽浓度范围的过氧化氢表现出优异的安培传感器性能，灵敏度为260 μA mM⁻¹ cm⁻²。作为一个应用实例，通过连接葡萄糖氧化酶（GOx），制备了一种对葡萄糖具有放大作用的生物传感器。该葡萄糖生物传感器在 - 0.1 V下对葡萄糖具有选择性测定能力，线性响应范围为5×10⁻⁶至1.5×10⁻² M，相关系数为0.997，响应时间<10 s。葡萄糖生物传感器的高灵敏度高达30 μA mM⁻¹ cm⁻²，检测限为3 μM。该生物传感器显示出快速响应、扩展的线性响应范围以及优异的重复性和稳定性。
