Matsuo K, Yamaguchi R, Yamazaki A, Tasaka H, Terasaka K, Totsuka M, Kobayashi K, Yukitake H, Yamada T
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki City, Japan.
Infect Immun. 1990 Dec;58(12):4049-54. doi: 10.1128/iai.58.12.4049-4054.1990.
In order to develop recombinant Mycobacterium bovis BCG into a useful multivaccine vehicle, we established a foreign antigen secretion system in mycobacteria in which an extracellular alpha antigen of Mycobacterium kansasii was utilized as a carrier. By using this system, a B-cell epitope (Glu-12-Leu-Asp-Arg-Trp-Glu-Lys-Ile-19) of human immunodeficiency virus type 1 p17gag, which was identified by a fusion protein-based method, has been successfully obtained from BCG along with the alpha antigen. This is the first report of expression and secretion of a foreign viral antigen from BCG. It is possible that the system can become a universal vaccination vehicle applicable to protection against various infectious diseases.
为了将重组牛分枝杆菌卡介苗开发成一种有用的多疫苗载体，我们在分枝杆菌中建立了一种外源抗原分泌系统，其中堪萨斯分枝杆菌的细胞外α抗原被用作载体。通过使用该系统，已成功从卡介苗中获得了基于融合蛋白法鉴定的人类免疫缺陷病毒1型p17gag的B细胞表位（Glu-12-Leu-Asp-Arg-Trp-Glu-Lys-Ile-19）以及α抗原。这是关于卡介苗表达和分泌外源病毒抗原的首次报道。该系统有可能成为适用于预防各种传染病的通用疫苗载体。
