博来霉素对经甲苯处理的大肠杆菌细胞中脱氧核糖核酸合成的影响。
Effect of bleomycin on deoxyribonucleic acid synthesis in toluene-treated Escherichia coli cells.
作者信息
Ross S L, Moses R E
出版信息
Antimicrob Agents Chemother. 1976 Feb;9(2):239-46. doi: 10.1128/AAC.9.2.239.
Abstract
The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.
摘要
抗生素博来霉素可刺激经甲苯处理的大肠杆菌细胞中的脱氧核糖核酸（DNA）合成。合成量的增加与博来霉素浓度呈线性关系。博来霉素刺激的DNA合成不依赖于复制，而是依赖于DNA聚合酶I。随着依赖于DNA聚合酶I的DNA合成增加，复制过程得以保留。博来霉素似乎对纯化的大肠杆菌DNA聚合酶I或II没有任何影响。我们的结果表明，博来霉素会导致细菌染色体产生切口，随后由DNA聚合酶I催化进行DNA合成。
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本文引用的文献
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Purification of bleomycins.博来霉素的纯化
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New antibiotics, bleomycin A and B.新型抗生素，博来霉素A和B。
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Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.对大肠杆菌中对DNA合成温度敏感的突变体的DNA聚合酶II和3的分析。
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Replication and repair of DNA in cells of Escherichia coli treated with toluene.用甲苯处理的大肠杆菌细胞中DNA的复制与修复
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Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.无细胞提取物中的纯化及DNA合成：DNA聚合酶II的特性
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A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1.大肠杆菌的一种新的DNA聚合酶活性。I. 大肠杆菌polA1中存在的该活性的纯化及特性
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