Huth Karin C, Jakob Franz M, Saugel Bernd, Cappello Christian, Paschos Ekaterini, Hollweck Regina, Hickel Reinhard, Brand Korbinian
Department of Restorative Dentistry and Periodontology, Ludwig-Maximilians-University, Munich, Germany.
Eur J Oral Sci. 2006 Oct;114(5):435-40. doi: 10.1111/j.1600-0722.2006.00390.x.
Ozone has been proposed as an alternative antiseptic agent in dentistry based on reports of its antimicrobial effects in both gaseous and aqueous forms. This study investigated whether gaseous ozone (4 x 10(6) microg m(-3)) and aqueous ozone (1.25-20 microg ml(-1)) exert any cytotoxic effects on human oral epithelial (BHY) cells and gingival fibroblast (HGF-1) cells compared with established antiseptics [chlorhexidine digluconate (CHX) 2%, 0.2%; sodium hypochlorite (NaOCl) 5.25%, 2.25%; hydrogen peroxide (H(2)O(2)) 3%], over a time of 1 min, and compared with the antibiotic, metronidazole, over 24 h. Cell counts, metabolic activity, Sp-1 binding, actin levels, and apoptosis were evaluated. Ozone gas was found to have toxic effects on both cell types. Essentially no cytotoxic signs were observed for aqueous ozone. CHX (2%, 0.2%) was highly toxic to BHY cells, and slightly (2%) and non-toxic (0.2%) to HGF-1 cells. NaOCl and H(2)O(2) resulted in markedly reduced cell viability (BHY, HGF-1), whereas metronidazole displayed mild toxicity only to BHY cells. Taken together, aqueous ozone revealed the highest level of biocompatibility of the tested antiseptics.
基于臭氧在气态和水溶液形式下的抗菌作用报告，其已被提议作为牙科中的一种替代抗菌剂。本研究调查了气态臭氧（4×10⁶微克/立方米）和水溶液臭氧（1.25 - 20微克/毫升）与已确立的抗菌剂[葡萄糖酸氯己定（CHX）2%、0.2%；次氯酸钠（NaOCl）5.25%、2.25%；过氧化氢（H₂O₂）3%]相比，在1分钟时间内对人口腔上皮（BHY）细胞和牙龈成纤维细胞（HGF - 1）是否具有任何细胞毒性作用，并与抗生素甲硝唑在24小时内的作用进行比较。评估了细胞计数、代谢活性、Sp - 1结合、肌动蛋白水平和细胞凋亡情况。发现臭氧气体对两种细胞类型均有毒性作用。水溶液臭氧基本未观察到细胞毒性迹象。CHX（2%、0.2%）对BHY细胞具有高毒性，对HGF - 1细胞有轻微毒性（2%）和无毒性（0.2%）。NaOCl和H₂O₂导致细胞活力显著降低（BHY、HGF - 1），而甲硝唑仅对BHY细胞显示出轻微毒性。总体而言，水溶液臭氧在所测试的抗菌剂中显示出最高水平的生物相容性。
