低强度激光疗法可减轻脂多糖诱导的大鼠膈肌肌肉收缩力功能障碍并降低肿瘤坏死因子-α水平。
Low-level laser therapy can reduce lipopolysaccharide-induced contractile force dysfunction and TNF-alpha levels in rat diaphragm muscle.
作者信息
Aimbire F, Lopes-Martins R A B, Castro-Faria-Neto H C, Albertini R, Chavantes M C, Pacheco M T T, Leonardo P S L M, Iversen V V, Bjordal J M
机构信息
Research Group of Animal Experimental, IP & D UNIVAP R. Shishima Hifumi, 2911, 12240-000, São José dos Campos, SP, Brazil.
出版信息
Lasers Med Sci. 2006 Dec;21(4):238-44. doi: 10.1007/s10103-006-0405-y. Epub 2006 Oct 11.
Our objective was to investigate if low-level laser therapy (LLLT) could improve respiratory function and inhibit tumor necrosis factor (TNF-alpha) release into the diaphragm muscle of rats after an intravenous injection of lipopolysaccharide (LPS) (5 mg/kg). We randomly divided Wistar rats in a control group without LPS injection, and LPS groups receiving either (a) no therapy, (b) four sessions in 24 h with diode Ga-AsI-Al laser of 650 nm and a total dose of 5.2 J/cm2, or (c) an intravenous injection (1.25 mg/kg) of the TNF-alpha inhibitor chlorpromazine (CPZ). LPS injection reduced maximal force by electrical stimulation of diaphragm muscle from 24.15+/-0.87 N in controls, but the addition of LLLT partly inhibited this reduction (LPS only: 15.01+/-1.1 N vs LPS+LLLT: 18.84+/-0.73 N, P<0.05). In addition, this dose of LLLT and CPZ significantly (P<0.05 and P<0.01, respectively) reduced TNF-alpha concentrations in diaphragm muscle when compared to the untreated control group.
我们的目的是研究低强度激光疗法（LLLT）是否能改善呼吸功能，并抑制大鼠静脉注射脂多糖（LPS，5毫克/千克）后肿瘤坏死因子（TNF-α）向膈肌的释放。我们将Wistar大鼠随机分为未注射LPS的对照组，以及接受以下处理的LPS组：（a）不进行治疗；（b）在24小时内进行4次治疗，使用波长650纳米的砷化镓铝二极管激光，总剂量为5.2焦/平方厘米；或（c）静脉注射（1.25毫克/千克）TNF-α抑制剂氯丙嗪（CPZ）。LPS注射使通过电刺激膈肌产生的最大力量从对照组的24.15±0.87牛降低，但添加LLLT部分抑制了这种降低（仅LPS组：15.01±1.1牛，LPS + LLLT组：18.84±0.73牛，P<0.05）。此外，与未治疗的对照组相比，该剂量的LLLT和CPZ分别显著降低了膈肌中TNF-α的浓度（P<0.05和P<0.01）。
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