Ulrich J, Meier-Ruge W, Probst A, Meier E, Ipsen S
Division of Neuropathology, Institute of Pathology, Basel, Switzerland.
Acta Neuropathol. 1990;80(6):624-8. doi: 10.1007/BF00307630.
In 20 unselected autopsy cases tissue blocks from the hippocampus with adjacent entorhinal cortex and neocortex were stained for acetylcholinesterase (AChE). From five brains shown to have large numbers of senile plaques tissue, adjacent to that taken for AChE tissue blocks, was embedded in paraffin and sections were immunostained for the A4 protein. The morphological aspects were compared. Equivalent types of plaques and plaque-like structures were observed in the A4- and ACHE-stained sections. On selected tissue blocks from patients with many senile plaques two immediately adjacent cryostat sections were stained, one for AChE and one for A4 protein. The same individual plaques could be identified on the two sections. These findings suggest that high AChE activity is intimately associated with the process of A4 protein formation and accumulation in plaques and that this association already occurs at a very early stage of plaque formation.
在20例未经挑选的尸检病例中，对带有相邻内嗅皮质和新皮质的海马组织块进行乙酰胆碱酯酶（AChE）染色。从5个显示有大量老年斑的大脑中，在用于AChE组织块取材的相邻部位取组织，将其包埋于石蜡中，切片进行A4蛋白免疫染色。对形态学方面进行比较。在A4染色切片和ACHE染色切片中观察到了同等类型的斑块和斑块样结构。在有许多老年斑的患者的选定组织块上，取两个紧邻的冰冻切片，一个进行AChE染色，另一个进行A4蛋白染色。在这两个切片上可以识别出相同的单个斑块。这些发现表明，高AChE活性与A4蛋白在斑块中的形成和积累过程密切相关，并且这种关联在斑块形成的非常早期阶段就已经出现。
