Paraskevopoulos Charalampos, Bordenstein Seth R, Wernegreen Jennifer J, Werren John H, Bourtzis Kostas
Department of Environmental and Natural Resources Management, University of Ioannina, 2 Seferi St., 30100, Agrinio, Greece.
Curr Microbiol. 2006 Nov;53(5):388-95. doi: 10.1007/s00284-006-0054-1. Epub 2006 Oct 11.
Among the diverse maternally inherited symbionts in arthropods, Wolbachia are the most common and infect over 20% of all species. In a departure from traditional genotyping or phylogenetic methods relying on single Wolbachia genes, the present study represents an initial Multilocus Sequence Typing (MLST) analysis to discriminate closely related Wolbachia pipientis strains, and additional data on sequence diversity in Wolbachia. We report a new phylogenetic characterization of four genes (aspC, atpD, sucB, and pdhB), and provide an expanded analysis of markers described in previous studies (16S rDNA, ftsZ, groEL, dnaA, and gltA). MLST analysis of the bacterial strains present in 16 different Drosophila-Wolbachia associations detected four distinct clonal complexes that also corresponded to maximum-likelihood identified phylogenetic clades. Among the 16 associations analyzed, six could not be assigned to MLST clonal complexes and were also shown to be in conflict with relationships predicted by maximum-likelihood phylogenetic inferences. The results demonstrate the discriminatory power of MLST for identifying strains and clonal lineages of Wolbachia and provide a robust foundation for studying the ecology and evolution of this widespread endosymbiont.
在节肢动物中多样的母系遗传共生菌里，沃尔巴克氏体最为常见，感染了超过20%的所有物种。与依赖单个沃尔巴克氏体基因的传统基因分型或系统发育方法不同，本研究是对密切相关的嗜吞噬细胞无形体菌株进行区分的初步多位点序列分型（MLST）分析，以及关于沃尔巴克氏体序列多样性的额外数据。我们报告了四个基因（aspC、atpD、sucB和pdhB）的新系统发育特征，并对先前研究中描述的标记（16S rDNA、ftsZ、groEL、dnaA和gltA）进行了扩展分析。对存在于16种不同果蝇 - 沃尔巴克氏体组合中的细菌菌株进行的MLST分析检测到四个不同的克隆复合体，它们也对应于最大似然法确定的系统发育分支。在分析的16种组合中，有六种无法归入MLST克隆复合体，并且还显示与最大似然系统发育推断预测的关系存在冲突。结果证明了MLST在识别沃尔巴克氏体菌株和克隆谱系方面的鉴别能力，并为研究这种广泛存在的内共生菌的生态学和进化提供了坚实的基础。
