聚二甲基硅氧烷微流控技术的分析方法及其生物学应用。

The analytical approach to polydimethylsiloxane microfluidic technology and its biological applications.

作者信息

Kartalov Emil P, Anderson W French, Scherer Axel

机构信息

Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Norris Cancer Center, NOR6346, 1441 Eastlake Avenue, Los Angeles, California 90033, USA.

出版信息

J Nanosci Nanotechnol. 2006 Aug;6(8):2265-77. doi: 10.1166/jnn.2006.504.

DOI:10.1166/jnn.2006.504

PMID:17037833

Abstract

This review article discusses PDMS (polydimethylsiloxane) microfluidic devices and their biological applications. First, the already developed devices are classified from the viewpoints of underlying technology within a common logical framework comprising single-layer, multilayer, and integrated devices, as well as surface chemistry modifications of PDMS. Combinatorial techniques are applied to re-derive existing devices within this framework. Next, the relevant scales of both microfluidics and biology are compared, obtaining the promise and limitations of PDMS microfluidics. Finally, the body of work is reclassified in terms of addressed biological applications and compared to the standard methods in cellular and molecular biology, to offer insights for future devices and applications.

摘要

这篇综述文章讨论了聚二甲基硅氧烷（PDMS）微流控装置及其生物学应用。首先，在一个包含单层、多层和集成装置以及PDMS表面化学修饰的通用逻辑框架内，从基础技术的角度对已开发的装置进行分类。组合技术被应用于在这个框架内重新推导现有装置。接下来，比较了微流控和生物学的相关尺度，得出了PDMS微流控的前景和局限性。最后，根据所涉及的生物学应用对工作主体进行重新分类，并与细胞和分子生物学中的标准方法进行比较，为未来的装置和应用提供见解。