Song Li-Bing, Zhang Xing, Zhang Chang-Qing, Zhang Ying, Pan Zhi-Zhong, Liao Wen-Ting, Li Man-Zhi, Zeng Mu-Sheng
State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China.
Ai Zheng. 2006 Nov;25(11):1356-60.
BACKGROUND & OBJECTIVE: Except for the tight correlation to nasopharyngeal carcinoma, accumulating evidences show that Epstein-Barr virus (EBV) is correlated to other carcinomas. This study was to investigate the correlation of EBV to colorectal carcinoma in Chinese.
EBV DNA was detected by polymerase chain reaction (PCR) in 90 specimens of primary colorectal carcinoma and 25 specimens of corresponding adjacent non-cancerous tissue, with the primers covering 2 different regions of EBV genome, BamH I W fragment and latent membrane protein 1 (LMP1) exon 3. The expression of EBV nuclear antigen 1 (EBNA1) and LMP1 were determined by immunohistochemistry, and the expression of EBV-encoded RNAs (EBERs) was detected by in situ hybridization.
EBV LMP1 exon 3 and W fragment were detected in 27.7% and 32.2% of the 90 colorectal carcinoma specimens, which were significantly higher than the positive rate of EBV gene in the 25 adjacent non-cancerous tissues (4.0%, P<0.001). Of the 29 W fragment-positive tumors, 23 (79.3%) were EBNA1-positive, 1 (3.4%) was EBERs-positive; most EBNA1-positive cells were tumor cells with positive signals gathered in the nuclei. No expression of EBNA1 and EBERs were detected in the 8 W fragment-negative tumors. No expression of LMP1 was detected in all tumor specimens.
EBV infection might be associated with colorectal carcinoma in Chinese.
除了与鼻咽癌密切相关外，越来越多的证据表明，爱泼斯坦-巴尔病毒（EBV）与其他癌症也有关联。本研究旨在探讨EBV与中国人大肠癌的相关性。
采用聚合酶链反应（PCR）检测90例原发性大肠癌标本及25例相应癌旁非癌组织标本中的EBV DNA，引物覆盖EBV基因组的2个不同区域，即BamH I W片段和潜伏膜蛋白1（LMP1）外显子3。采用免疫组织化学法检测EBV核抗原1（EBNA1）和LMP1的表达，采用原位杂交法检测EBV编码RNA（EBERs）的表达。
90例大肠癌标本中，EBV LMP1外显子3和W片段的检出率分别为27.7%和32.2%，显著高于25例癌旁非癌组织中EBV基因的阳性率（4.0%，P<0.001）。在29例W片段阳性肿瘤中，23例（79.3%）EBNA1阳性，1例（3.4%）EBERs阳性；大多数EBNA1阳性细胞为肿瘤细胞，阳性信号聚集在细胞核中。8例W片段阴性肿瘤中未检测到EBNA1和EBERs的表达。所有肿瘤标本中均未检测到LMP1的表达。
EBV感染可能与中国人大肠癌有关。
